靶向MagT1的siRNA对乳腺癌细胞增殖和凋亡的影响  

Effects of siRNA targeting MagT1 gene on proliferation and apoptosis of breast cancer cells

作　　者：滕飞翔[1] 成海龙[1] 凌存保[1] 吴隽松[1] 郝玲[1] 张栋梁[1] 陈鹤林[1] 

机构地区：[1]盐城卫生职业技术学院临床医学院,江苏省224005

出　　处：《江苏医药》2015年第20期2376-2378,共3页Jiangsu Medical Journal

基　　金：盐城市医学科技发展计划项目(YK2012037)

摘　　要：目的研究小干扰RNA(siRNA)沉默镁离子转运蛋白1(MagT1)的表达对人乳腺癌细胞系MCF-7增殖和凋亡的影响。方法将MCF-7细胞分为MagT1siRNA实验组(A组)、siRNA阴性对照组(B组)和空白细胞对照组(C组)。采用RT-PCR和Western blot法分别检测MagT1mRNA和蛋白表达,MTT法检测细胞增殖情况,流式细胞术检测细胞凋亡和半胱天冬氨酸蛋白酶9(Caspase-9)表达情况。结果与B组和C组相比,A组MagT1mRNA和蛋白表达降低,Caspase-9表达增加,细胞增殖率降低,细胞凋亡率增加(P<0.05)。结论沉默MagT1表达能抑制乳腺癌细胞增殖,促进细胞凋亡;线粒体信号转导途径参与了细胞凋亡过程。Objective To investigate the effects of magnesium transporter 1（MagT1）silenced by small interference RNA（siRNA）on the proliferation and apoptosis of human breast cancer cell line MCF-7.Methods MCF-7cells were divided into three groups of A（transfected with MagT1siRNA）,B（transfected with siRNA negative control）and C（blank control）.The mRNA and protein expressions of MagT1 were detected by RT-PCR and Western blot,respectively.The cell proliferation was measured by MTT assay.The cell apoptosis and expression of cystein asparate proteinase（Caspase-9）were determined by flow cytometry.Results Compared with groups of B and C,the expressions of MagT1 mRNA and protein were decreased while expression of Caspase-9was increased in group A（P〈0.05）.The cell proliferation rate was lower and cell apoptosis rate was higher in group A than those in groups of B and C（P〈0.05）.Conclusion Silencing MagT1 expression can inhibit MCF-7cell proliferation and promote its apoptosis,in which mitochondrial signaling pathway participates.

关 键 词：镁离子转运蛋白1 小干扰RNA 乳腺癌 

分 类 号：R737[医药卫生—肿瘤]