miR-17-5P对肺癌细胞株A549、SPCA-1和GLC-82的增殖及侵袭能力的影响  

The impose of miR-17-5P over cell proliferation and invasion in lung cancer cells A549,SPCA-1 and GLC-82

作　　者：孟丽娟[1] 王峻[1] 樊卫飞[1] 蒲骁麟[1] 许菊青 王琳[1] 杨民[1] 刘福银[1] 

机构地区：[1]江苏省老年医院血液肿瘤科,江苏南京210024

出　　处：《中国肿瘤外科杂志》2015年第5期282-286,共5页Chinese Journal of Surgical Oncology

基　　金：江苏省基础研究计划(BK2011842);江苏省"六大人才高峰"项目(2012-WSN-045)

摘　　要：目的探讨miR-17-5P对肺癌细胞株A549、SPCA-1及GLC-82增殖及侵袭能力的影响及其分子机制。方法利用化学合成的miR-17-5P mimics及miR-17-5P inhibitor转染A549、SPCA-1及GLC-82肺癌细胞株,通过MTT、Transwell小室迁移实验、Boyden小室侵袭实验观察过表达及抑制表达miR-17-5P后细胞迁移、侵袭能力及生长的变化;Western blot检测细胞周期、上皮间叶转变(EMT)标志物相关蛋白。结果 MTT法显示,与对照组比较,过表达miR-17-5P组细胞生长速度增加(P<0.001);miR-17-5P表达抑制后,细胞生长速度降低(P<0.001)。Transwell及Boyden实验显示过表达组细胞穿过聚碳酸酯膜的数量多于对照组(P<0.001),抑制表达后细胞穿膜数减少,并少于对照组(P<0.001)。Western blot示,与对照组比较,过表达miR-17-5P的A549、SPCA-1及GLC-82细胞中,CCND1、N-CA、Vimentin表达上调,抑制miR-17-5P的A549、SPCA-1及GLC-82细胞中,CCND1、N-CA、Vimentin表达下调。结论 miR-17-5P可能通过上调CCND1、Vimentin、N-CA促进肺癌细胞株A549、SPCA-1及GLC-82的增殖、迁移及侵袭。Objective The aim of this study was to investigate the effect and molecular mechanism of miR-17-5P over cell proliferation and invasion in lung cancer cells A549 , SPCA-1 and GLC-82. Methods miR-17-5P mimics and inhibitor were synthesized and transfected into lung cancer cells A549, SPCA-1 and GLC-82. The abilities of cell growth,migration and invasion were examined by MTT,Transwell, and Boyden chamber. Westen blot was used to test the expression of cell cycle and EMT-associated proteins. Results Compared with the control group,over expression of miR-17-5P improved A549, SPCA-1 and GLC-82 cells growth and number across polycarbonate membrane（P 〈0. 001）. While miR-17-5P expression was inhibited, cells growth and number across polycarbonate membrane were improved（P〈0. 001）. Over expression of miR-17-5P upregulate the expression of CCND1,Vimentin and N-CA in A549, SPCA-1 and GLC-82. Meantime, expression of CC-ND1,Vimentin and N-CA was downgraded when expression of miR-17-5P was inhibited. Conclusion miR-17-5P may facilitate cell proliferation, migration and invasion in A549 , SPCA-1 and GLC-82, by which mechanism is related to the improvement of expression of CCNDl,Vimentin and N-CA.

关 键 词：miR—1715P 肺癌 增殖 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]