姜黄素对海人酸致痫大鼠PI3K/AKt/mTOR通路调节作用的实验研究  

Regulation Effects of Curcumin on PI3K/Akt/mTOR Pathway in Kainate- induced Epileptic Rats

作　　者：王娜 佟凤芝 曾常茜[2] 

机构地区：[1]辽宁省大连市第五人民医院,大连116021 [2]大连大学医学院,辽宁省生物有机重点实验室,大连116622

出　　处：《中国中医药科技》2015年第5期534-536,549,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：大连市科学技术基金计划项目(2008J21JH005)

摘　　要：目的:通过检测海人酸致痫大鼠海马神经元PI3K、AKt和m TOR的表达,探讨姜黄素对癫痫大鼠神经元保护的分子机制。方法:采用海人酸注射诱导大鼠癫痫造模,造模前用姜黄素干预7天。免疫组化方法检测大鼠海马神经元PI3K、AKt、m TOR蛋白表达,采用RT-PCR技术检测大鼠海马神经元PI3K、AKt、m TOR mRNA表达。结果:免疫组化结果显示,海人酸组神经元PI3K、AKt、m TOR蛋白表达较正常组降低(P<0.05),姜黄素组神经元的PI3K、AKt、m TOR蛋白表达较海人酸组增加(P<0.05)。RT-PCR结果显示,海人酸组海马神经元PI3K、AKt、m TOR mRNA表达水平与正常组比较显著降低(P<0.05),姜黄素组海马神经元PI3K、AKt、m TOR mRNA表达水平与海人酸组比较显著增高(P<0.05)。结论:姜黄素能够保护海人酸致痫大鼠的神经元损伤,其分子机制可能与上调PI3K/AKt/m TOR表达有关。Objective： To investigate the molecular mechanism for Curcumin to protect neurons through detecting the expressions of PI3 K,Akt and m TOR of neurons in kainic acid- induced epileptic rats. Methods：Models were built by kainate injection,Curcumin was given rats for 7 days before modeling. Immunohistochemical technique was used to detect the expressions of PI3 K,Akt and m TOR proteins of neurons in kainic acid- induced epileptic rats. RT- PCR technique was used to detect the expressions of PI3 K,Akt and m TOR mRNA of neurons in kainic acid- induced epileptic rats. Results： The expression of PI3 K,Akt and m TOR protein decreased obviously in kainic acid group compared with those in normal group（ P〈0. 05）,the expressions of PI3 K,Akt and m TOR protein increased obviously in Curcumin group compared with those in kainic acid group（ P〈0. 05）. The results of RT- PCR technique showed that the expressions of PI3 K,AKt,m TOR mRNA decreased obviously in kainic acid group compared with those in normal group（ P〈0. 05）,the expressions of PI3 K,AKt,m TOR mRNA increased obviously in Curcumin group compared with those in kainic acid group（ P〈0. 05）. Conclusion： Curcumin inhibits neurons damage in kainic acid- induced epileptic rat and the molecular mechanism maybe related to upregulating the expressions of PI3 K,AKt and m TOR proteins and mRNA.

关 键 词：癫痫 姜黄素 海马 神经元 PI3K/AKt/m TOR 基因表达 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]