模拟动脉栓塞条件下肝细胞癌化疗药敏评估  

Evaluation on the chemosensitivity of hepatocellular carcinoma during mimetic transcatheter arterial embolization

作　　者：李强[1] 朱林忠[2] 杨仁杰[2] 林虎[1] 敖国昆[1] 

机构地区：[1]解放军第309医院放射科,北京100091 [2]北京大学肿瘤医院介入科,100091

出　　处：《中华肝胆外科杂志》2015年第10期695-698,共4页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨肝癌细胞（HepG2细胞系）在缺氧缺养内环境下对5种肝动脉化疗栓塞（TACE）术中常用化疗药物单药及双药联合的敏感性。方法HepG2细胞分别在含化疗药的4种浓度缺氧缺养培养液中培养2、4、6、24h。MTT法测定各自抑瘤率。所用5种化疗药物分别为：吡柔比星、顺铂、丝裂霉素C、奥沙利铂、5-氟尿嘧啶。结果在4种缺氧缺养条件下均敏感的药物是：吡柔比星、丝裂霉素C、奥沙利铂。在氧浓度为5％、10％、25％的培养液中，5种化疗药作用24h的抑瘤率均明显高于2h的抑瘤率。在氧浓度50％培养液中，5种化疗药2时间亚组间的抑瘤率差异无统计学意义。与单药比较，双药联合组抑瘤率并无明显增加。结论吡柔比星、丝裂霉素C、奥沙利铂在不同缺氧缺养条件下均对HepG2细胞具有有效的毒性作用。在中重度缺氧缺养条件下，延长药物作用时间可以提高药物的抑瘤作用。而双药联合不能增强疗效。Objective To investigate whether 5 different chemotherapeutic drugs and their combina- tion of either two drugs could further promote the inhibition on the cell growth of HCC cell line （ HepG2 ） in vitro in the hypoxic and hyponutritional culture medium （HHCM） mimicking the different scenarios of tran- scatheter arterial chemoembolization （TACE）. Methods The cells were treated by 5 drugs for 2 h, 4 h, 6 h and 24 h, which include epirubicin （ EPI ）, cisplatin （ DDP ）, mitomycin-C （ MMC ）, oxaliplatin （OXA） and 5-fluorouracil （5-FU） in four concentrations of HHCM （5% , 10% , 25% and 50% ） mimic- king the scenarios during TACE and the cell viability was assessed using 3-（4,5-dimethyhhiazol-2-yl）-2,5- diphenyltetrazolium bromide （MTF） assay. The combinations of dual drugs treated for 24 h were also tested. Results The sensitive drugs with inhibition rates more than 30% were EPI, MMC and OXA in 4 different concentrations of HHCM. The sensitivity of the drugs treated for 24 h was significantly increased compared with that for2 h in 5%, 10% and 25% HHCM. The dual combinations did not increase the ehemosensitivi- ty of HepG2 ceils. Conclusions EPI, MMC and OXA exhibited cytotoxie activity against HepG2 ceils in various hypoxia and hyponutrition states. Prolonging the exposure time could increase the sensitivity of drug in HHCM, and the combination of dual drugs cannot enhance the cytotoxic effect.

关 键 词：肝细胞癌 经导管动脉化疗栓塞 药物敏感试验 缺氧 

分 类 号：R730.53[医药卫生—肿瘤]