miR-153在卵巢癌中的表达及其生物学功能  

Expression and biological function of miR-153 in ovarian cancer

作　　者：韩小妮 周静[1] 孙玉芳[1] 戴星[1] 胡鸣[1] 焦榕芳[1] 方念[2] 

机构地区：[1]南昌大学第四附属医院妇产科,330003 [2]南昌大学第四附属医院消化内科,330003

出　　处：《中华临床医师杂志（电子版）》2015年第18期56-58,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：江西省卫生厅科技计划(20131101);江西省科技支撑计划(20142BBG70076)

摘　　要：目的分析mi R-153在卵巢癌中的表达,并探讨mi R-153的生物学作用。方法荧光定量PCR法检测卵巢癌组织及细胞中mi R-153的表达水平;转染mi R-153类似物(mimics)至SKOV3细胞,MTT法、平板克隆实验及流式细胞仪分别检测mi R-153对细胞增殖及凋亡的影响。结果 mi R-153在卵巢癌组织及细胞中的表达显著下调;转染mi R-153 mimics后细胞生长受到明显抑制,克隆数目减少,细胞早期凋亡率显著增加(P<0.01)。结论 mi R-153在卵巢癌中表达下调;过表达mi R-153可显著抑制SKOV3细胞增殖,促进细胞凋亡。Objective This study aims to analyze the miR-153 expression profile in ovarian cancer, explore the biological functions of miR-153. Methods The relative expression of miR-153 in ovarian cancer tissues and cancer cell lines were detected by real-time quantitative RT-PCR. Introduction of miR-153 mimics to SKOV3 cells. The cell proliferation ability were detected by MTT assay and colony formation, and cell apoptosis was detected by flow cytometry. Results The expression of miR-153 was significantly decreased in ovarian cancer tissues and cell lines; introduction of miR-153 mimics into the SKOV3 cells inhibited cell proliferation, colony formation ability and induced apoptosis （P〈0.01）. Conclusion miR-153, which is significantly downregulated in ovarian cancer tissues and cell lines, inhibits cell proliferation and induces apoptosis.

关 键 词：卵巢肿瘤 细胞增殖 细胞凋亡 miR-153 

分 类 号：R737.31[医药卫生—肿瘤]