耐酚异养硝化菌的筛选及其对苯酚降解特性的研究  

Isolation and identification of a phenol heterotrophic nitrifier and its characteristics for the degradation of phenol

作　　者：纪南南 武文丽[1] 颜家保[1] 曹晓诗 胡茜茜[1] 吴优[1] 

机构地区：[1]武汉科技大学化学工程与技术学院,湖北武汉430081

出　　处：《化学工程师》2015年第10期1-3,共3页Chemical Engineer

基　　金：武汉科技大学研究生创新创业基金重点项目(JCX0006);武汉科技大学国家级大学生创新创业训练计划项目(201310488009)

摘　　要：从炼油废水活性污泥中筛选出1株能降解苯酚的异养硝化菌株Y3,经生理生化实验及16S r DNA序列分析鉴定其为鲍曼不动杆菌(Acinetobacter baumannii)。该菌降解苯酚的适宜温度为30℃、摇床转速为150r·min-1、培养基初始p H值为为8.0;在优化条件下,菌株Y3经11.5h的培养,可将质量浓度为508.47mg·L-1的苯酚完全降解;菌株Y3对1000mg@L-1的苯酚,55h的降解率为100%。A phenol heterotrophic nitrifier Y3 was isolated from the activated sludge of petrochemical wastewa- ter treatment system. It was identified as a member of the genus Acinetobacter baumannii according to its morphy- ological and physiological properties and the analysis of its 16S rDNA gene sequence. The optimal conditions for phenol removing are as follows： temperature 30℃, skaking speed 150r.min-1, pH 8.0. Under this conditions, phe- nol can be decreased from 508.47mg .L-1 to 0mg.L-1 after 11.5h, and phenol could be decomposed completely when phenol concentration was 1000mg.L-1.

关 键 词：异养硝化 苯酚 氨氮 炼油废水 

分 类 号：X172[环境科学与工程—环境科学]