上皮性卵巢癌组织中FOXC1 mRNA表达变化及其启动子区甲基化情况观察  

Observation of the FOXC1 mRNA changes in ovarian cancer and its methylation status in promoter region

作　　者：李倩[1] 任琛琛[1] 杨立[1] 薛景戈 白杨[1] 张娟[1] 黄玲[1] 

机构地区：[1]郑州大学第三附属医院,郑州450052

出　　处：《山东医药》2015年第38期21-23,共3页Shandong Medical Journal

基　　金：河南省科技发展计划项目(20100511)

摘　　要：目的观察卵巢上皮性肿瘤中FOXC1 mRNA表达变化及其启动子区甲基化情况。方法选择30例上皮性卵巢癌(A组)、14例交界性卵巢上皮性肿瘤(B组)和21例良性卵巢上皮性肿瘤患者(C组),取其手术切除卵巢组织标本,用RT-PCR法检测FOXC1 mRNA,用甲基化特异聚合酶链反应方法(MSP)结合琼脂糖凝胶电泳检测FOXC1基因启动子区甲基化状态及频率。结果 A、B、C组FOXC1 mRNA相对表达量分别为0.38±0.16、0.61±0.18、1.19±0.17,A组低于B组,B组低于C组(P均<0.05)。A组卵巢癌组织中FOXC1 mRNA相对表达量与临床分期及分化程度无关,与淋巴结转移有关(P<0.05)。A、B、C组卵巢组织中FOXC1基因启动子区甲基化频率分别为66.7%、50.0%、9.5%,A组高于B组,B组高于C组(P均<0.05)。A组FOXC1基因启动子区甲基化、未甲基化者FOXC1 mRNA相对表达量分别为0.32±0.14、0.45±0.17,P<0.05。结论上皮性卵巢癌组织中FOXC1 mRNA表达下降,FOXC1 mRNA表达与上皮性卵巢癌淋巴结转移有关。FOXC启动子区甲基化状态与其mRNA表达抑制相关。Objective To study the correlation among transcriptional expression and promoter methylation of FOXC1 gene in epithelial ovarian tumors and its clinical characteristics. Methods Methylation-specific PCR was used to detect the methylation of FOXC1 gene promoter Cp G island in 65 patients who underwent operation for epithelial ovarian tumors,reverse transcription PCR was performed to detect the expression of FOXC1 mRNA. Result FOXC1 mRNA was showed in all epithelial ovarian tumor samples. The relative level of FOXC1 mRNA was 1. 19 ± 0. 17 in benign tumors,0. 61 ± 0. 18 in borderline tumors and 0. 38 ± 0. 16 in malignant tumors respectively. It was significantly lower in ovarian cancer than that in benign tumor specimens,and was significantly lower in borderline tumor than that in benign tumor too（ P〈0. 05）. The expression of FOXC1 was not correlated with clinical characteristics,such as clinical stage,histological differentiation.However there were lower expression level of FOXC1 mRNA in patients with lymph node metastasis than that of patients without lymph node metastasis. The methylation rate of the FOXC1 gene promoter Cp G island in ovarian cancer was higher than those in borderline tumor and the benign tumor,P〈0. 05. Conclution The expression of FOXC1 is closely correlated with the oncogenesis and progress of ovarian cancer. Promoter methylation may contribute to the low level of mRNA expression.

关 键 词：上皮性卵巢癌 叉头框转录因子 FOXC1基因 DNA甲基化 基因表达 

分 类 号：R737.31[医药卫生—肿瘤]