血管抑制因子对脉络膜新生血管抑制作用的实验研究  

An experimental research on the inhibitory effect of recombinant human Vasostatin for choroidal neovascularization

作　　者：李沭岩[1] 吴雅臻[2] 张磊[1] 

机构地区：[1]上海市杨浦区中心医院眼科,200090 [2]吉林大学第二临床学院眼科

出　　处：《中华眼外伤职业眼病杂志》2015年第10期739-743,共5页Chinese Journal of Ocular Trauma and Occupational Eye Disease

基　　金：上海市卫生局资助课题（20114357）;上海市卫生局资助课题（20134419）

摘　　要：目的：探讨重组人血管抑制因子（ vasostatin）抑制激光诱导的小鼠脉络膜新生血管形成（CNV）的作用。方法 C57BL/6J成年小鼠30只（30只眼）,均用右眼,通过Nd： YAG激光眼底光凝诱导小鼠形成脉络膜新生血管,随机分为对照组、低剂量组和高剂量组,每组各10只小鼠。在眼底Nd：YAG激光光凝后立即分别给予A组（对照组）每只小鼠球后注射10μl生理盐水；B组（低剂量组）各组每只小鼠球后注射血管抑制因子10μl（2μg/μl）；C组（高剂量组）注射10μl（4μg/μl）。在光凝后7 d及14 d通过荧光素眼底血管造影（ FFA）评价CNV的发生率。结果治疗组在光凝后7 d及14 d CNV发生率及各光凝斑荧光素渗漏程度均比对照组轻。光凝后7 d,A组脉络膜新生血管的发生率为68%,B组和C组脉络膜新生血管的发生率分别为56%、48%,与A组比较,差异均有统计学意义（P〈0.05）。光凝后14 d,A组脉络膜新生血管的发生率为72%,B组和C组脉络膜新生血管的发生率分别为50%、38%,与A组比较,差异均有统计学意义（P〈0.01）。结论血管抑制因子可以有效抑制激光诱导的小鼠实验性CNV。通过Nd：YAG激光照射脉络膜损伤成功地诱导了小鼠CNV模型,这种CNV模型制作方法可用于CNV的发病机制及治疗方法的研究。Objective To investigate the effects of recombinanthuman Vasostatin on the inhibition of Nd：YAG laser induced mice choroidal neovascularization （CNV）. Methods In 30 adult C57BL/6J mice（30 eyes）, choroid angiogenesis was induced by Nd： YAG laser fundus photocoagulation in the right eye. The mice were randomly divided into control group, low dose group and high dose group with 10 mice in each group. Immediately after the Nd：YAG laser photocoagulation, retrobulbar injection of 10 μl saline was peformed in all mice in group A （ control group）;Mice in treatment group was retrobulbarly injected with 10μl of recombinant human Vasostatin at the concentration of 2μg/μl in group B and 4μg/μl in group C. The choroidal neovascularization was evaluated by fundus fluorescence angiography 7 and 14 days after photocoagulation. Results In the treatment group, the incidence of CNV and spot fluorescein leakage were lighter than that of control group 7 d and 14 d after photocoagulation. Seven days after photocoagulation, choroidal neovascularization incidence was 68% in group A. The incidence rates of choroid neovascularization in group B and group C were 56% and 48% respectively. There was statistically significant difference （P〈0. 05）. Fourteen days after photocoagulation, the choroid neovascularization incidence was 72% in group A. It was 50% and 38% in group B and group C respectively, which was statistically significant different from group A （P〈0. 01）. Conclusion Vasostatin can effectively suppress experimental laser-induced mouse CNV. Choroidal injury by the Nd：YAG laser can successfully induce mouse CNV model.

关 键 词：脉络膜 新生血管形成 血管抑制因子( Vasostatin) 疾病模型 小鼠 

分 类 号：R773.4[医药卫生—眼科]