刺玫果提取物中总三萜酸的定性定量分析研究  

Qualitative and Quantitative Analysis of Total Triterpenoid Acids in Fruit Extracts of Rosa davurica Pall.

作　　者：钟方丽[1] 王晓林[1] 杨扬[1,2] 

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022 [2]吉林大学化学学院,吉林长春130012

出　　处：《林产化学与工业》2015年第5期135-142,共8页Chemistry and Industry of Forest Products

基　　金：吉林省教育厅资助项目(吉教科合字[2013]第318号)

摘　　要：建立刺玫果提取物中总三萜酸的定性定量分析方法。采用TLC法对刺玫果提取物总三萜酸中的熊果酸进行定性分析;采用UV法对刺玫果提取物中的总三萜酸进行了定量分析;采用HPLC法对刺玫果提取物总三萜酸中的齐墩果酸、熊果酸进行了定量分析。结果表明,UV法中齐墩果酸在1.75~15.75 mg/L范围内与吸光度有良好的线性关系（r=0.999 1）,平均加样回收率为101.60%（RSD1.23%）;HPLC法中齐墩果酸、熊果酸在0.1~1.1μg（r=0.999 7）、0.024~0.768μg（r=0.999 9）线性关系良好,平均加样回收率分别为100.24%（RSD 1.44%）、100.54%（RSD 0.45%）。3批刺玫果提取物中总三萜酸平均为8.62%,齐墩果酸平均为0.273%,熊果酸平均为0.534%。结果表明,所建立的刺玫果提取物中总三萜酸的定性分析和定量分析方法可用于该提取物的质量控制。The qualitative and quantitative analysis methods of total triterpenoid acids（ TTA） in fruit extracts of Rosa davurica Pall. were established. Both UV and HPLC methods were applied to determine the content of TTA,oleanolic acid and ursolic acid. TLC method was used to accomplish the qualitative identification of ursolic acid in fruit extracts. The concentration of oleanolic acid showed good linear relationship with absorbance at the range of 1. 75- 15. 75 mg / L（ r = 0. 999 1） and the average recovery was 101. 60%（ RSD 1. 23%） in quantitative analysis of TTA with UV method. On the other hand,oleanolic acid and ursolic acid showed good linear relationship at the range of 0. 1- 1. 1 μg（ r = 0. 999 7） and 0. 024- 0. 768 μg（ r = 0. 999 9）respectively and the average recoveries of them were 100. 24%（ RSD 1. 44%） and 100. 54%（ RSD 0. 45%） by HPLC method.The average contents of TTA,oleanolic acid,and ursolic acid of three batches of fruit extracts were 8. 62%,0. 273%,and0. 534%,respectively. The experimental results showed that the content determination and TLC qualitative identification methods established could be applied to control of the qualitative and quantitative analysis of fruit extracts of R. davurica.

关 键 词：刺玫果 总三萜酸 齐墩果酸 熊果酸 

分 类 号：TQ35[化学工程]