苦参碱对人胆管癌细胞系QBC939增殖及凋亡的影响及其机制  

Effect of matrine on proliferation and apoptosis of human cholangiocarcinoma cell line QBC939 and its mechanisms

作　　者：周欢[1] 雷宇[1] 梁凯[1] 郭晴[1] 

机构地区：[1]广西医科大学附属肿瘤医院药学部,南宁市530021

出　　处：《广西医学》2015年第8期1065-1068,共4页Guangxi Medical Journal

摘　　要：目的探讨苦参碱在体外对人胆管癌细胞系QBC939增殖抑制和凋亡的影响及其机制。方法采用MTT法检测不同浓度苦参碱对QBC939细胞增殖抑制率,Annexin V-FITC/PI双染法检测细胞凋亡率,罗丹明123检测线粒体膜电位,Western Blot法检测Cyto-c、Bcl-2、BAX和活化caspase-3蛋白表达的变化情况。结果随着苦参碱浓度的增大,QBC939细胞增殖的抑制率升高(P<0.001);随着处理时间的延长,苦参碱对QBC939细胞增殖的抑制率升高(P<0.001),苦参碱对QBC939细胞增殖的抑制作用,呈剂量和时间依赖性。随着苦参碱浓度的升高,早期凋亡率、晚期凋亡率及总凋亡率均升高(P<0.001),苦参碱对QBC939细胞凋亡的诱导作用,且呈剂量依赖性。罗丹明123检测显示药物处理浓度越大,弱荧光峰比例越高(P<0.001)。与对照组比较,0.5 mg/ml、1.0 mg/ml、1.5 mg/ml组的Cyto-c、BAX及活化caspase-3的表达逐渐增强,Bcl-2的表达降低。结论苦参碱对人胆管癌细胞系QBC939的增殖具有明显的抑制作用并能诱导凋亡,其机制可能与调节线粒体通路的Bcl-2/BAX蛋白表达,激活下游的caspase-3有关。Object ive To explore the effect of matrine on antiproliferation and apoptosis of human cholangiocarcinoma cell line QBC939 in vitro and its mechanisms.Methods The antiproliferation rate of QBC939 cells induced by different concentrations of matrine was detected using MTT assay,the apoptosis rate of QBC939 cells was assessed by Annexin V-FITC/PI staining method,and the mitochondrial membrane potential was analyzed by Rhodamine 123 assay.The expressions of Cyto-c,Bcl-2,BAX and activated caspase-3 proteins were detected by Western Blot.Results The antiproliferation rate of QBC939 cells increased with the increasing concentration of matrine or treatment time（P〈0.001）,and the antiproliferation effect of matrine on QBC 939 cells showed a doset-ime dependence.The early apoptosis rate,late apoptosis rate and total apoptosis rate increased with the increasing concentration of matrine （P〈0.001）,and the apoptosis effect of matrine on QBC939 cells showed dose dependence.Rhodamine 123 assay revealed that the proportion of weak fluorescence peak increased with the concentration of matrine increasing（P〈0.001）.In the groups of concentrations of 0.5,1.0 and 1.5 mg/ml,the expressions of Cyto-c, BAX and activated caspase-3 increased gradually,while the expression of Bcl-2 decreased compared to the controls.Conclusion Matrine has obvious inhibitory effect on the proliferation of QBC939 cells,and can induce the apoptosis of the cells,whose mechanisms may be related to the regulation of the expressions of Bcl-2/BAX proteins in the mitochondrial pathway and the activation of downstream caspase-3.

关 键 词：胆管癌 苦参碱 凋亡 增殖 线粒体 膜电位 

分 类 号：R735.8[医药卫生—肿瘤]