小鼠胃肠动力分型障碍模型探索研究  

Study of Classification of Gastrointestinal Motility Disorder in Mice Model

作　　者：王吉娥 刘童婷 徐萌[1] 黄秀深[1] 陈继兰[1] 

机构地区：[1]成都中医药大学,四川成都611137 [2]四川好医生药业集团有限公司,四川成都610075

出　　处：《辽宁中医杂志》2015年第10期2005-2007,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(30973713)

摘　　要：目的:初步探索小鼠胃肠动力分型障碍模型。方法:对正常小鼠胃肠动力分析,以小鼠胃排空率和小肠推进率为指标分别建立小鼠胃肠动力评价标准,将小鼠胃肠动力分为抑制、稳定和亢进三种类型。采用综合造模法建立小鼠胃肠动力障碍模型,分别于造模2、4、6、8、10 d后处死小鼠,测定小鼠胃残留率和小肠推进率,确定小鼠胃肠动力障碍模型的造模时间,选择适合实验的小鼠胃肠动力模型评价指标。结果:正常小鼠胃肠动力标准建立:以胃残留率为标准建立模型评价,小鼠胃残留率在(60.00±15.86)%为稳定型,胃残留率在(98.39±2.71)%为抑制型,胃残留率在(21.86±9.13)%为亢进型;以小肠推进率为标准建立模型评价,小鼠胃残留率在(56.93±21.89)%为稳定型,胃残留率在(8.48±7.20)%为抑制型,胃残留率在(97.30±3.12)%为亢进型。建立小鼠胃肠动力障碍模型,以第2、4、6天作为小鼠胃肠动力障碍模型的造模时间,以小肠推进率为标准建立的模型评价作为小鼠胃肠动力障碍造模方法的评价方法。结论:正常小鼠中存在胃肠动力障碍问题,以胃残留率和小肠推进率为标准分别建立小鼠胃肠动力障碍模型评价标准,以小肠推进率为标准建立的模型评价作为小鼠胃肠动力障碍造模方法的评价方法。Objective： To preliminarily study the classification of gastrointestinal motility disorder in mice model. Methods： Gastrointestinal motility in normal mice was analyzed. The rate of gastric emptying and intestinal propulsion rate indicators were as the indexes for establishing the mice gastrointestinal motility evaluation criteria and gastrointestinal motility of mice was divided into three types： suppression,stability and hyperthyroidism. Integrated modeling method was used to establish the mice gastrointestinal motility disorder model. Respectively,2,4,6,8,10 days after modeling,the mice were sacrificed for determining the residual rate in mice stomach and small intestine. We determined the gastrointestinal motility disorder modeling time for this experiment and chose the corresponding index for gastrointestinal motility evaluation. Results： Normal mice＇ s gastrointestinal motility standards were established. The gastric residual rate was as the standard model evaluation. The rate of gastric residual was（ 60. 00 ± 15.86） % for stability,（ 98. 39 ± 2. 71） % for suppression and（ 21. 86 ± 9. 13） % for hyperthyroidism. The small intestine propulsion rate was as the index for evaluationg. The rate of gastric residual was（ 56. 93 ± 21. 89） % for stability,（ 8. 48 ± 7. 20） % for inhibitory and（ 97. 30 ± 3. 12） % for hyperthyroidism. Integrated modeling method was established by mice gastrointestinal motility disorder model for 2,4,6 days as the modeling time and the small intestine propulsion rate as the evaluation standards. Conclusion： Normal mice have gastrointestinal motility disorder. By taking the gastric residual rate and the small intestine propulsion rate as the standards,the mice gastrointestinal motility disorder models were set up and the latter one was used for the evaluation of the modeling of mice gastric motility disorder.

关 键 词：胃肠动力分型障碍模型 胃排空率 肠推进率 

分 类 号：R573.9[医药卫生—消化系统]