裸鼠阳虚证乳腺癌骨转移模型的建立  

Establishment of Nude Mouse Model of Yang Deficiency and Bone Metastasis of Breast Cancer

作　　者：龚辉[1] 黄立中[1] 毛丹[1] 冯磊[1] 刘吉勇[1] 

机构地区：[1]湖南中医药大学,湖南长沙410007

出　　处：《新中医》2015年第11期200-203,共4页New Chinese Medicine

基　　金：高等学校博士学科点专项科研基金(2011432311006);湖南省自然科学基金(12JJ5055);湖南省研究生创新课题(CX2014B353)

摘　　要：目的:采用病、证复合模型的方法建立裸鼠阳虚证乳腺癌骨转移模型。方法:采用臀部肌肉注射氢化可的松的方法建立裸鼠阳虚证模型;采用左心室注射乳腺癌细胞MDA-MB-231悬液建立裸鼠乳腺癌骨转移模型;动态观察各组裸鼠生存情况、体重变化,进行X线摄像观察,测定环磷酸腺苷(c AMP)、环磷酸鸟苷(c GMP)、血清甲状腺三碘原氨酸(T3)等值。结果:肌注氢化可的松后,裸鼠出现畏寒肢冷、活动减少、精神萎靡、皮毛少光泽、饮食减少、大便清稀等阳虚表现,且c AMP明显下降、c GMP明显升高、c AMP/c GMP值下降,T3值明显升高;左心室注射乳腺癌细胞悬液后裸鼠出现不同程度的骨转移,且经X线摄像检查确诊。结论:对裸鼠肌注氢化可的松可成功建立阳虚证模型;左心室注射法建立乳腺癌骨转移模型的成功率较高。Objectives= To establish nude mouse model of Yang deficiency and bone metastasis of breast cancer by the combination of disease and syndrome method. Methods. Intramuscular injecting hydrocortisone to establishe the Yang deficiency syndrome model, and injecting breast cancer MDA-MB-231 cells suspension into the left Ventricular to establishe bone metastasis model. Nude mice survival, the body weight changes, the X-ray imaging, and the levels of cycric adenosine monophosphate（cAMP）, cyclic guanosine monophosphate（cGMP）, testosterone（T）3 were observed. ResuJts： After injecting hydrocortisone, the nude mice represented Yang deficiency syndrome, such as being cold, reducing activity, being apathetic, less shiny fur, less eating, thin stool, and the levels of cAMP being decreased, cGMP being increased, cAMP/cGMP being decreased, T3 being increased obviously. After injecting breast cancer cells suspension into the left Ventricular, the nude mice appeared different degrees of bone metastasis, and the X-ray imaging examination and histopathological examination confirmed the diagnosis. Conclusion： Hydrocortisone injection can establish nude mouse model of Yang deficiency successfully. Injecting breast cancer cells suspension into the left Ventricular can establish the nude mouse model of bone metastasis of breast cancer in a high successful rate.

关 键 词：乳腺癌 骨转移 阳虚证 裸鼠模型 

分 类 号：R-332[医药卫生]