米油对实验性肠屏障损伤的保护作用  

THE PROTECTIVE EFFECTS OF RICE OIL ON EXPERIMENTAL INTESTINAL BARRIER DAMAGE

作　　者：杨军红[1] 司萍[2] 林莹[1] 田曦[1] 李萍[1] 陈颖新[1] 

机构地区：[1]天津市儿童医院营养科,天津300074 [2]天津市儿童医院检验科,天津300074

出　　处：《营养学报》2015年第5期451-455,共5页Acta Nutrimenta Sinica

基　　金：天津市卫生局科技基金(2013KZ039)

摘　　要：目的 探讨米油对实验性大鼠肠黏膜损伤的保护作用及其机制。方法 SD雄性大鼠80只,体质量180~200g,随机分为5组,每组16只,A组为正常对照组,其余4组造腹泻模型。B组作为单纯腹泻组,C、D、E组灌胃内毒素。C组灌胃生理盐水,D组灌胃5%米油,E组灌胃蒙脱石散。观察大鼠的一般情况及腹泻次数、造模后D3和D7肠道SIg A、肠黏膜形态学变化。结果 A组腹泻次数显著低于其他各组（P〈0.01）;D和E组比较没有统计学差异（P〉0.05）,且从D3开始腹泻次数明显减少;D和E组与B和C组腹泻次数比较均有统计学意义（P〈0.01）。病理显示,D7各组黏膜形态均不同程度的好于D3;D7时,A、D、E组明显好于B和C组。C组SIg A的浓度于造模后D3明显低于其他各组（P〈0.05）;D7时,D和E组SIg A的浓度明显高于其他各组（P〈0.01）;而B和C组SIg A则显著下降（P〈0.01）。结论 米油能提高腹泻大鼠肠道免疫功能,有利于修复小肠的组织结构和功能,提高急性腹泻大鼠的治疗效果。Objective To investigate the protective effects of rice oil on experimental intestinal barrier damage in rats. Methods Eighty SD rats, weighing 180-200g, were randomly divided into 5 groups. There were 16 rats in each group. Group A was normal control group and group B was pure diarrhea group. Groups C,D,E were given endotoxins, normal saline（NS）, 5% rice oil, montmorillonite power by gavage respectively. Then, the changes general conditions and diarrhea times, intestinal SIgA levels, intestinal mucosal morphology were observed. Results Diarrhea times of group A was significantly lower than other groups （P〈0.01）. Group D had no statistical difference compared with group E, and diarrhea times decreased significantly from the 3rd day （P〉0.05）. Diarrhea times of group D and E had statistical significance compared with group B and C （P〈0.01）. Pathologic examination revealed that mucosal morphology at the day 7 were better than at day 3. Groups A,D,E were significantly better than groups B, C at day 7. SIgA concentration of group C was significantly lower than other groups after 3 days （P〈0.05）. SIgA sconcentration of groups D,E were significantly higher than other groups （P〈0.01） while that of groups B,C were significantly declined （P〈0.01）. Conclusion Rice oil can improve intestinal immune function, repair small intestine tissue structure and function in rats with acute diarrhea.

关 键 词：大鼠 腹泻 米油 肠黏膜 

分 类 号：R285.5[医药卫生—中药学]