长链非编码RNA在乳腺癌患者外周血与癌组织中的表达  

Expression of long non-coding RNA in peripheral blood and carcinoma tissue of breast cancer patients

作　　者：吕明明[1] 许楠[1,2] 王凤良[1] 陈飞[1] 陆迅 陆澄[1,2] 

机构地区：[1]南京医科大学附属南京市妇幼保健院乳腺病科,210004 [2]南京中医药大学第一临床医学院,210046 [3]南京金陵中学,210005

出　　处：《中华乳腺病杂志（电子版）》2015年第4期242-246,共5页Chinese Journal of Breast Disease(Electronic Edition)

基　　金：国家自然科学基金资助项目(81172501);南京市医学科技发展项目(YKK12103)

摘　　要：目的比较分析长链非编码RNA(lncRNA)在乳腺癌组织与外周血中的表达情况,筛选出参与乳腺癌发生、复发及转移过程中重要的lncRNA。方法利用芯片技术分别检测并筛选乳腺癌组织与外周血差异表达的lncRNA及mRNA,应用生物信息学方法,包括基因本体论(gene ontology,GO)和Pathway分析,分析乳腺癌组织与外周血中lncRNA及mRNA的表达谱。分析得到乳腺癌外周血与癌组织中共同表达与特异表达的lncRNA,并且进一步分析差异基因可能调控的相关通路,进而发现参与乳腺癌发生发展和转移过程的lncRNAs。结果笔者发现在乳腺癌组织和外周血中共同上调的lncRNA有7条(包括RP11-707G14.7、RP11-771K4.1、XLOC_013658、RP11-201A3.1、RP11-298H24.1、BM151951、XLOC_011403),共同下调的lncRNA有12条(LOC100506035、PWRN1、MAGI2-AS3、RP11-67L3.2、CTC-454M9.1、AC017006.2、AC002456.2、RP11-430B1.2、RP11-945C19.4、RP11-839D17.3、CTA-407F11.8、LINC00420)。GO和Pathway分析结果表明在乳腺癌患者外周血和组织中均差异表达的基因主要参与调控免疫反应与TGF-β通路等。结论 lncRNA在乳腺癌组织和外周血中呈差异表达,可能参与到乳腺癌的发生、发展以及复发转移中,有可能成为临床诊断与治疗乳腺癌的新靶点。Objective To compare the expression of long non-coding RNA （ lncRNA ） in both peripheral blood and carcinoma tissues of breast cancer patients and reveal the important lncRNAs that may participating in the development, recurrence and metastasis of breast cancer. Methods Microarray chip was used to detect and screen the differentially expressed lncRNA and mRNA in carcinoma tissue and peripheral blood of breast cancer patients. lncRNA and mRNA expression profiles were analyzed by bioinformatics tools, including gene ontology （ GO ） and Pathway analysis. The differentially expressed and commonly expressed lncRNAs both in peripheral blood and carcinoma tissues were detected. Moreover, we further analyzed the relevant pathways regulated by differentially expressed genes, as well as lncRNAs participating in the development and metastasis of breast cancer. Results There were 7 up-regulated lncRNAs （ including RP11-707G14. 7,RP11-771K4. 1,XLOC 013658,RP11-201A3. 1,RP11-298H24. 1,BM151951,XLOC 011403） and 12 down-regulated lncRNAs in both carcinoma tissue and peripheral blood （ including LOC100506035, PWRN1, MAGI2-AS3, RP11-67L3. 2, CTC-454M9. 1, AC017006. 2, AC002456. 2, RP11-430B1. 2, RP11-945C19. 4, RP11-839D17. 3, CTA-407F11. 8, LINC00420 ）. GO and Pathway analysis indicated that differentially expressed genes in both peripheral blood and carcinoma tissues of breast cancer patients were mainly involved in the regulation of immune response and TGF-β pathway. Conclusion lncRNAs were differentially expressed genes in both peripheral blood and carcinoma tissues of breast cancer patients, which may be involved in the development, recurrence and metastasis of breast cancer, regarded as possible potential targets for clinical diagnosis and treatment.

关 键 词：乳腺肿瘤 分子诊断技术 芯片分析技术 基因表达谱 微RNAS 

分 类 号：R737.9[医药卫生—肿瘤]