强直性脊柱炎湿热痹阻证血清差异蛋白组学研究  

Differential Serum Proteomic Analysis of Ankylosing Spondylitis Patients with Damp-heat Arthralgia Spasm Syndrome

作　　者：孙志岭[1] 徐骁[1] 王玲[2] 王富强[2] 许志洋[2] 纪伟[3] 

机构地区：[1]南京中医药大学护理学院,江苏南京210023 [2]南京医科大学分析测试中心,江苏南京210029 [3]南京中医药大学附属医院,江苏南京210029

出　　处：《南京中医药大学学报》2015年第5期412-415,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81173158);江苏省高校"青蓝工程"(2014);江苏省高校优秀中青年教师赴境外研修项目(2013)

摘　　要：目的应用蛋白质组学双向电泳和质谱技术,开展强直性脊柱炎湿热痹阻证证候学研究。方法对强直性脊柱炎湿热痹阻证、寒湿痹阻证、正常对照共3组各30例血清,分别进行双向凝胶电泳,软件分析获取湿热痹阻证组和其他2组的差异蛋白质点,二级质谱鉴定获得差异蛋白。应用Western blot验证差异蛋白。结果获得7个湿热痹阻证的共同差异蛋白点,经质谱鉴定和SwissProt数据库检索,获得Fibulin-1、二肽基肽酶1、激肽原1、白细胞介素17F突变型、甘露糖结合蛋白C等5个强直性脊柱炎湿热痹阻证差异蛋白,涉及胞外基质蛋白、蛋白酶类、凝血因子、细胞因子、转运蛋白等。Western blot验证甘露糖结合蛋白C的表达结果与蛋白组学检测结果一致。结论这些蛋白可能涉及强直性脊柱炎湿热痹阻证的发病和病理过程,为进一步研究湿热痹阻证实质提供了依据。OBJECTIVE Proteomic technique was applied to analyze ankylosing spondylitis AS patients with damp-heat arthralgia spasm syndrome.METHODS Serum proteins of AS patients with damp-heat arthralgia spasm syndrome groupn=30 was compared with that of normal control group n = 30 and AS patients with cold-dampness arthralgia spasm syn-drome groupn =30 respectively by two-dimensional gel electrophoresisthe common differential protein spots of damp-heat arthralgia spasm syndrome were analyzed by the second order MS.The validation of the differential protein was performed u-sing Western blot.RESULTS Seven kinds of common differential protein spots were selected and five kinds of proteins were i-dentified by mass spectrometry.They were fibulin 1dipeptidy lpeptidase 1kininogen 1mutant IL 17Fmannose-binding protein C and related to extracellular matrix proteinproteasecoagulation factorcytokinetransport proteinand so on.Re-sults of Western blot were consistent with the proteomic results.CONCLUSION These proteins are implicated in the patho-genesis and pathological processes of AS with damp-heat arthralgia spasm syndromeand provide a basis for further studies of the essence of the syndrome in traditional Chinese medicine.

关 键 词：湿热痹阻证 强直性脊柱炎 双向凝胶电泳 质谱 血清 

分 类 号：R255.6[医药卫生—中医内科学]