膀胱癌细胞系T24侧群细胞的分离及初步鉴定  

Isolation and identification of side population cells in human bladder cancer cell line T24

作　　者：殷波[1] 张露丹[2] 刘岗[1] 王鹏[1] 张超[1] 田宝勇[1] 宋永胜[1] 

机构地区：[1]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110004 [2]中国医科大学

出　　处：《现代肿瘤医学》2015年第22期3211-3214,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81372725)

摘　　要：目的:检测和分选人类膀胱癌细胞系T24中的侧群(side population,SP)细胞,并初步鉴定其癌干细胞特性,为癌干细胞的分离纯化奠定基础。方法:采用荧光激活细胞分选(FACS)技术,分选得到T24细胞中的侧群细胞,并检测其比例。采用反转录聚合酶链反应(RT-PCR)技术检测侧群细胞中ABCG2的表达情况;继而将侧群细胞培养于无血清培养基中,观察形成悬浮肿瘤细胞球的能力。结果:流式细胞分选结果显示:T24细胞中侧群细胞的比例约为3.6%。和对应的母系T24细胞相比,T24侧群细胞的ABCG2表达增高。培养于无血清培养基中的侧群细胞成簇生长,并形成肿瘤细胞球。结论:人类膀胱癌细胞系T24中存在具有癌干细胞特性的侧群细胞。Objective： To identify the side population（ SP） cells from human bladder cancer cell line T24 and characterize the cancer stem cell-like phenotype of these SP cells,which lays the foundation for the isolation and purification of cancer stem cells. Methods： Fluorescence activated cell sorting（ FACS） was applied to isolate SP cells from T24 cell line. Reverse transcription-polymerase chain reaction（ RT-PCR） was used to detect the expression of ABCG2 in T24 SP cells and parental T24 cells. The isolated SP cells were cultured in serum-free medium to observe the capacity of growing into tumor spheres. Results： SP cells were isolated from T24 cells by flow cytometry,with a proportion of 3. 6%. A higher expression of ABCG2 in SP cells was detected by RT-PCR in contrast to parental T24 cells. When cultured in serum-free medium,SP cells grew into sphere-like clusters / tumor spheres compared to the parental T24 cells. Conclusion： These multiple lines of evidence suggest that T24 cell line contained SP cells with cancer stem cell properties.

关 键 词：膀胱癌 侧群细胞 癌干细胞 ABCG2 

分 类 号：R730[医药卫生—肿瘤] R737.14[医药卫生—临床医学]