大肠癌细胞HCT-15肿瘤干细胞样细胞分离及成瘤性比较  

Isolation of cancer stem like cells from colorectal cancer cell HCT-15 and tumorigenicity comparison

作　　者：邢亚楠[1] 邓鹏[1] 徐惠绵[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤外科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2015年第22期3226-3228,共3页Journal of Modern Oncology

基　　金：辽宁省科技计划项目(编号:2011225019)

摘　　要：目的:依据人大肠癌细胞HCT-15的表面标志物,分离其中的干细胞亚群。建立裸鼠体内原代大肠癌荷瘤模型,比较各亚群肿瘤体积和质量。方法:利用免疫磁珠分选技术,分离其中CD133/CD44干细胞亚群。分选得到CD133+CD44+、CD133+CD44-亚群分别接种于裸鼠体内,并观察肿瘤大小和质量。结果:CD133+CD44+和CD133+CD44-细胞亚群成功的从HCT-15细胞中分离出来。接种CD133+CD44+细胞的裸鼠形成的肿瘤体积[(2.76±0.22)cm3]和质量[(5.2±0.21)g]明显高于接种CD133+CD44-细胞裸鼠的肿瘤体积与质量[(1.56±0.34)cm3,(3.4±0.18)g](P<0.05)。结论:CD44阳性的大肠癌肿瘤干细胞成瘤能力明显高于CD44阴性的大肠癌肿瘤干细胞。Objective： According to the surface markers of HCT-15 cancer cell,to isolate the stem cancer cells.To build colorectal cancer xenografts models and compare the tumor size and weight of SCID hairless congenic mice.Methods： We isolated the stem cancer cells from human colorectal cancer cell HCT-15 by magnetic microbeads.CD133＋CD44＋and CD133＋CD44-stem cancer cells were subcutaneously inoculated to SCID hairless mice,then compared the tumor size and weight. Results： CD133＋CD44＋and CD133＋CD44-cancer stem cells were isolated successfully from HCT-15 cells. The tumor size derived from CD133＋CD44＋cancer stem cells [（ 2. 76 ± 0. 22）cm3]was larger than CD133＋CD44-ones [（ 1. 56 ± 0. 34） cm3]（ P 0. 05）. And the weight [（ 5. 2 ± 0. 21） g]was also higher than negative ones [（ 3. 4 ± 0. 18） g]（ P 0. 05）. Conclusion： The tumorigenicity of CD44 positive colorectal stem cancer cells was significantly higher than negative ones.

关 键 词：大肠癌 CD133 CD44 成瘤性 肿瘤干细胞 

分 类 号：R735.34[医药卫生—肿瘤]