肾癌中E-cadherin甲基化状态的检测及其与β-catenin表达的关系  

The relationship between the methylation status of E-cadherin gene and β-catenin expression in renal cell carcinoma

作　　者：刘彬[1] 张爱莉[1] 赵志红[1] 倪晓辰[1] 

机构地区：[1]河北医科大学第四医院泌尿外科,河北石家庄050011

出　　处：《现代肿瘤医学》2015年第21期3122-3127,共6页Journal of Modern Oncology

摘　　要：目的:研究肾癌(renal cell carcinoma,RCC)中E-钙黏蛋白(E-cadherin)甲基化状态的检测及其与β-链蛋白(β-catenin)表达的关系。方法:应用甲基化特异性聚合酶链反应(methylation specific PCR,MSP)技术检测53例肾癌组织及相应癌旁正常组织中E-cadherin基因的甲基化状态。免疫组织化学SP法检测53例肾癌组织及相应癌旁正常组织中E-cadherin和β-catenin蛋白的表达。结果:在基因水平和蛋白水平,E-cadherin甲基化与β-catenin的蛋白表达呈负相关(r=-0.361,P<0.05);在蛋白水平,E-cadherin与β-catenin呈正相关(r=0.716,P<0.001),在基因水平和蛋白水平,E-cadherin甲基化与蛋白表达呈负相关(r=-0.296,P<0.05)。结论:E-cadherin和β-catenin可能共同参与了包括肾癌的发生、发展、侵袭、转移等一系列病理过程。肾癌组织E-cadherin基因甲基化的发生可能与β-catenin抑制表达有关,可通过β-catenin与Wnt传导通路产生交互协同作用,影响β-catenin的细胞内分布,共同影响肾癌的侵袭转移进程。同时,提示Wnt通路在肾癌中处于激活状态,表明该传导通路的异常激活与肾癌的发生关系密切。Objective：To investigate the relationship between the methylation status of E - eadherin gene and β - catenin expression in renal cell carcinoma. Methods：Methylation specific PCR （MSP） method was used to examine the methylation status of E - eadherin gene in tumors and corresponding non - cancerous renal tissue. Immunohisto- chemistry was used to examine the expression of E - eadherin and β - eatenin in 53 specimens of renal cell carcinoma tissue and 53 specimens of adjacent non - tumor renal tissue. Results： Between gene level and protein level, there were negative correlations between E -cadherin gene methylation and expression of β -catenin protein in renal cell carcinoma tissues （ r = -0. 361 ,P 〈 0.05 ）. There was a positive correlation between E - cadherin protein and β - catenin protein in renal cell carcinoma tissues （r = 0. 716, P 〈 0. 001 ）. Between gene level and protein level, there were negative correlations between E - eadherin gene methylation and expression of E - cadherin protein in renal cell carcinoma tissues （ r = - 0. 296 ,P 〈 0.05 ）. Conclusion ： E - eadherin and β - catenin maybe collectively participa- ted in a series of pathological progression （tumorigenesis, progression,invasion and metastasis） of renal cell carcino- ma. Besides participating cell adhesion, E - eadherin might interact with Wnt signaling pathway. It had effect on the invasion and metastasis of renal cell carcinoma through changing the intracellular distribution of β - eatenin. It indica- ted that this signaling pathway was involved in tumorigenesis.

关 键 词：肾癌 E-钙黏蛋白 Β-链蛋白 免疫组织化学 DNA甲基化 

分 类 号：R737.11[医药卫生—肿瘤]