Foxp3在肺癌细胞中的表达及对肺癌细胞侵袭和转移的影响  

The Influences of Foxp3 on Cell Growth, Migration and Invasion of Lung Cancer Cells

作　　者：林群英[1] 樊克兴[2] 林国盛[1] 郭丽景[1] 陈国欢[1] 林娟[1] 

机构地区：[1]莆田学院附属医院呼吸内科,福建莆田351100 [2]第二军医大学肿瘤研究所,上海200433

出　　处：《莆田学院学报》2015年第5期11-15,共5页Journal of putian University

基　　金：福建省自然科学基金资助项目(2013J01390)

摘　　要：研究Foxp3在三株人肺癌细胞系A549、NCI-H209和95-D中的表达情况及其对肺癌细胞增殖、侵袭和转移的影响。实时荧光定量RT-PCR和Western-blot方法检测Foxp3在三株肺癌细胞系中的m RNA和蛋白水平;构建Foxp3干扰RNA,并瞬时转染A549细胞;MTS细胞增殖实验、细胞侵袭及划痕实验研究Foxp3对A549细胞体外增殖、侵袭以及转移能力的影响。结果:在三株肺癌细胞系中,相对于正常肺上皮细胞,Foxp3的m RNA和蛋白水平均呈高表达,其中在A549细胞中表达水平最高。细胞免疫荧光检测发现Foxp3主要定位于肺癌的细胞质中。瞬转Foxp3干扰RNA至A549细胞,可以明显促进肺癌细胞的侵袭和转移,但是对肺癌细胞的增殖无显著影响。此外,还发现干扰Foxp3表达可以显著上调MMP-9和u PA的表达水平。结果表明,Foxp3在肺癌细胞中呈高表达,其在肺癌中可能发挥抑癌作用。The paper investigated the expression of Foxp3 on lung cancer cell line A549, NCI-H209 and 95-D, and the role of Foxp3 on tumor cell growth, migration and invasion in vitro. The expression of Foxp3 in human lung cancer cell lines was determined by Real-time RT-PCR and western-blotting. si RNA of Foxp3 was transfected into A549 cells transietly, and cell proliferation, invasion and migration were determined by MTS assay, matrigel transwell assay, cell wound healing assay. High m RNA and protein expressions of Foxp3 were detected in three lung cancer cell lines.Amongst them, the levels of Foxp3 in A549 cells showed highest. Moreover, it observed that Foxp3 protein was located in cytoplasmic, not nuclear by cellular immunofluorescence staining. Furthermore, silence of Foxp3 expression in A549 cells enhanced cell migration and invasion, but could not affect the proliferation. In addition, it was found that silence of Foxp3 expression could increase the m RNA and protein levels of MMP-9 and u PA. Foxp3 were highl expression in lung cancer cells, and might be a onco-suppressor gene.

关 键 词：FOXP3 肺癌细胞 细胞增殖 侵袭 转移 

分 类 号：R734.2[医药卫生—肿瘤]