骨髓间充质干细胞移植脑梗死模型后的CT、MRI特征分析  

Bone marrow mesenchymal stem cell transplantation for cerebral infarction: CT and MRI characteristics

作　　者：杨廷双[1] 

机构地区：[1]天津医科大学总医院滨海医院放射科,天津市300480

出　　处：《中国组织工程研究》2015年第36期5795-5799,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究表明骨髓间充质干细胞可通过多种途径发挥促进脑组织功能恢复的作用。目的:分析骨髓间充质干细胞移植脑梗死模型大鼠的CT、MRI特征。方法:将40只脑梗死模型大鼠随机分为2组,梗死组通过尾静脉注射1 m L PBS、移植组通过尾静脉注射1 m L细胞浓度为2.0×109 L-1的细胞悬液。于移植后1,2,3周进行神经功能缺损评分(m NSS),评价神经功能恢复情况;于移植后的6 h,1,3,5,7 d对各组大鼠行CT及MRI扫描,观察脑梗死区TIWI、T2WI、FLAIR、DWI序列的信号改变特征,梗死体积大小。测量脑梗死区域T1WI、T2WI、FLAIR、DWI序列的信号强度比(SIR)及其相对变化率(?SIR),并与正常对侧相应解剖区域进行比较。结果与结论:移植后1,2,3周,移植组的神经功能缺损评分明显低于梗死组(P<0.05);移植后3,5,7 d时移植组脑梗死体积较梗死组显著减少(P<0.05),移植组T1WI序列SIR均明显高于梗死组,T2WI、FLAIR序列SIR较梗死组显著降低,差异有显著性意义(P<0.05);移植后7 d时移植组DWI序列SIR较梗死组明显降低(P<0.05)。移植组T1WI序列?SIR与梗死组比较差异无显著性意义(P>0.05),移植组T2WI、FLAIR及DWI序列?SIR较梗死组显著增高,差异有显著性意义(P<0.05)。结果显示MRI可显示大脑任意角度的切面像,对骨髓间充质干细胞移植治疗脑梗死的疗效评价起重要作用。BACKGROUND： Studies have shown that bone marrow mesenchymal stem cells can play a catalytic role in brain function recovery through a variety of ways. OBJECTIVE： To analyze the CT and MRI features of rats with cerebral infarction undergoing bone marrow mesenchymal stem cell transplantation. METHODS： Forty rats with cerebral infarction were randomized into two groups： model group was treated with 1 mL PBS and transplantation group was injected with 2.0×10^9/L cell suspension. Modified neurological severity score was detected at 1, 2, 3 weeks after transplantation for neurological function evaluation. CT and MRI scans were performed at 6 hours, 1, 3, 5, 7 days after transplantation to observe the changing features of T1 WI, T2 WI, FLAIR, DWI sequences in the infracted area as well as infarction size. Signal intensity ratios（SIR） of T2 WI, FLAIR, DWI sequences and relative rates of change（△SIR） were determined and compared with the normal values. RESULTS AND CONCLUSION： After transplantation 1, 2 and 3 weeks, the modified neurological severity scores were significantly lower in the transplantation group than the model group（P〈0.05）. After transplantation 3, 5, 7 days, the infracted size was reduced significantly in the transplantation group compared with the model group（P〈0.05）. Compared with the model group, SIR value of T1 WI sequence was higher, but the SIR values of T2 WI and FLAIR sequences were lower in the transplantation（P〈0.05）. There were significant differences in the △SIR values of T2 WI, FLAIR and DWI（P〈0.05） rather than T1WI（P〉0.05） between the two groups. These findingsindicate that MRI can show the brain sections at any angle, and play an important role to assess the therapeutic efficacy of bone marrow mesenchymal stem cell transplantation on cerebral infarction.

关 键 词：干细胞 移植 骨髓间充质干细胞 大鼠 脑梗死 CT MRI 特征分析 细胞移植 

分 类 号：R394.2[医药卫生—医学遗传学]