非小细胞肺癌肿瘤组织干细胞标志物CD133的表达  

The expression of CD133 in non-small cell lung cancer

作　　者：都瑾[1] 高建辉[2] 韩纪昌[1] 李红兵[1] 程相树[3] 

机构地区：[1]河南大学淮河医院呼吸内科,河南省开封市475000 [2]新乡医学院医学检验学院,河南省新乡市453000 [3]河南大学医学院,河南省开封市475000

出　　处：《中国组织工程研究》2015年第36期5816-5820,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:非小细胞肺癌肿瘤组织中干细胞标志物CD133表达会呈现出一定的变化,并与疾病的发生和发展存在一定的联系。目的:探讨非小细胞肺癌肿瘤组织干细胞标志物CD133的表达情况,分析其与临床病理因素以及预后之间的关系。方法:收集135例非小细胞肺癌组织标本和60例正常肺组织标本。采用免疫组织化学染色法检测两组标本CD133的表达,并分析非小细胞肺癌肿瘤组织中CD133蛋白的表达与临床病理因素之间的关系。结果与结论:1非小细胞肺癌组CD133蛋白阳性表达率显著高于正常对照组(P<0.05)。2CD133蛋白阳性表达与非小细胞肺癌患者年龄、性别以及肿瘤大小、肿瘤位置、组织学类型无关(P>0.05);随着非小细胞肺癌肿瘤组织分化越差,CD133蛋白阳性表达呈现出不断上升趋势(P<0.05);CD133蛋白阳性表达率在不同临床分期以及淋巴结有无转移之间差异也有显著性意义(P<0.05)。3CD133以及TNM分期是非小细胞肺癌患者预后独立影响因素(P<0.05),CD133蛋白表达阳性组中位生存时间显著短于阴性组(P<0.05)。以上结果表明CD133参与非小细胞肺癌的发生和发展以及浸润和转移,对疾病的进展以及预后判断有重要的临床意义。BACKGROUND： The CD133 expression in non-small cell lung cancer shows some changes, which is definitely related to the occurrence and development of diseases. OBJECTIVE： To investigate the expression of CD133 in non-small cell lung cancer, and to analyze its relationship with clinical pathological factors and prognosis. METHODS： Non-small cell lung cancer tissue specimens from 135 cases were collected, and normal lung tissue specimens from 60 cases were set as normal control group. Immunohistochemical staining was used to detect CD133 expression in two groups, and the relationship between the expression of CD133 protein and the clinical pathological factors was analyzed. RESULTS AND CONCLUSION：（1） The positive expression of CD133 protein in the non-small cell lung cancer group was significantly higher than that in the normal control group（P〈0.05）.（2） CD133 protein expression had no association with age, gender, tumor size, tumor location, histological type（P〉0.05）, and CD133 protein expression was significantly increased with the differentiation of non-small cell lung cancer（P〈0.05）. The positive expression rate of CD133 protein was significantly different between different clinical stages and lymph node metastasis（P〈0.05）.（3） CD133 and TNM staging were independent prognostic factors for non-small cell lung cancer（P〈0.05）, and the median survival time was significantly shorter in the positive group than in thenegative group（P〈0.05）. The results indicate that CD133 is involved in the occurrence, development, infiltration and metastasis of non-small cell lung cancer, and it has important clinical significance for the disease progression and prognosis.

关 键 词：干细胞 肿瘤干细胞 非小细胞肺癌 干细胞标志物 CD133蛋白 

分 类 号：R394.2[医药卫生—医学遗传学]