有机溶剂辅助封闭式电喷雾离子源的设计及其在痕量蛋白质组样品分析中的应用  

Design of Organic Vapor Assisted Tight Ion Source and Its Application in Trace Analysis of Proteomic Samples

作　　者：朱旭东[1,2] 周愿[1] 梁振[1] 梁玉[1] 张丽华[1] 朱一心 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所,中科院分离分析化学重点实验室,大连116023 [2]中国科学院大学,北京100049 [3]浙江好创生物技术有限公司,杭州311121

出　　处：《分析化学》2015年第10期1433-1438,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21235005;21205115)资助项目~~

摘　　要：将康达效应应用于电喷雾离子源,设计了气氛可调康达效应电喷雾离子源（Coanda effect electrospray ionization source,CEESI）。考察了不同饱和蒸气压、不同极性的有机溶剂对标准肽段在质谱上信号响应的影响。结果表明,辅助气中添加乙醇后,标准肽段质谱信号提高约1000倍,而且对于1 ng BSA酶解产物,蛋白序列覆盖率提高约10%。在最佳条件下,采用nano-HPLC-CEESI-LTQ XL MS系统,实现了6,10,20和50 ng He La细胞全提蛋白质酶解产物的定性分析,与常用的开放式nano-ESI相比,肽段和蛋白质的可鉴定数目分别提高20%~360%和20%~200%。本研究表明,采用CEESI源有利于痕量蛋白质组样品的高灵敏度分析。A novel Coanda effect electrospray ionization source（ CEESI） was designed and applied in the analysis of the digests of bovine serum albumin（ BSA） and proteins extracted from He La cells. Firstly,the effects of various organic solvents on the mass spectrometry signal of a standard peptide were investigated.With ethanol vapor,the signal of standard peptide was increased by about 1000 fold,and the sequence coverage of 1 ng of BSA was improved by about 10%. Finally,under the optimized conditions,6,10,20 and50 ng of He La cell tryptic digests were qualitatively analyzed with nano-HPLC-CEESI-LTQ XL MS system. In comparison with commercial nano-ESI,the number of identified peptides and proteins were increased by20%- 360% and 20%- 200%,respectively,demonstrating that the CEESI ion source is promising in the in-depth profiling of trace proteomic samples.

关 键 词：康达效应 电喷雾离子源 蛋白质组 痕量分析 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657[轻工技术与工程—食品科学与工程]