新疆维吾尔族和汉族大学生尿液代谢轮廓差异分析  

Metabolite Profiling of Human Urine from Uygur and Han Undergraduates Population in Xinjiang

作　　者：宋培培[1] 马玉花[1] 韩萍[1] 刘洋 王吉德[1] 封顺[1] 

机构地区：[1]新疆大学化学化工学院,乌鲁木齐830046 [2]沈阳彦程生物制品有限公司,沈阳110000

出　　处：《分析化学》2015年第10期1565-1568,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(Nos.21075105;21165017);新疆维吾尔自治区教育厅支撑项目(No.XJEDU2010I0)~~

摘　　要：以新疆两个主要世居民族(维吾尔族和汉族)大学生人群尿样为研究对象,采用气相色谱-质谱联用技术对35名维吾尔族和37名汉族大学生人群尿样进行分析,考察两民族大学生尿液代谢轮廓间的差异。采用正交信号校正-偏最小二乘法判别分析结果表明,维吾尔族和汉族大学生人群尿样代谢轮廓存在着明显差异,聚类明显。实验发现和鉴定出9种差异代谢物,其中7种为内源性代谢物,2种为外源性代谢物。研究表明,相关代谢组学研究可从源头避免或减少非疾病因素引入的差异变量,除年龄、性别等因素外,还需充分考虑族别因素的影响。The aim of this study was to investigate the inter-individual variability of the metabolic urinary profile between Uygur and Han healthy populations,the largest population in Xinjiang. Totally 72 urine samples（ 35 Uygur and 37 Han undergraduates） were collected and analyzed based on a gas chromatographymass spectrometric platform. The data were analyzed by orthogonal signal correction filtered partial leastsquares discriminant analysis to screen variations reflecting metabolic characteristics between the two groups.Totally 9 metabolites were differentially expressed between two groups. Among which,seven compounds were endogenous metabolites,and the other two metabolites were exogenous. The results showed that non-disease specific factors should be diminished or decreased to improve the accuracy and reliability of relative biomarkers in multi-ethnic co-existed region,and the factor of ethic should be taken into accounts seriously besides age,sex.

关 键 词：代谢组学 气相色谱-质谱 维吾尔族 汉族 尿液 

分 类 号：R446.12[医药卫生—诊断学]