DNase I对金黄色葡萄球菌生物膜形成的影响  

Effect of DNase I on biofilm formation of Staphylococcus aureus

作　　者：徐巧玲 孙凤军[2] 冯伟[2] 刘晓[3] 刘亚巍 

机构地区：[1]总参警卫局卫生保健处,北京100017 [2]第三军医大学西南医院药剂科,重庆400038 [3]北京医院消化科,北京100005

出　　处：《南方医科大学学报》2015年第9期1356-1359,共4页Journal of Southern Medical University

摘　　要：目的研究DNase I对金黄色葡萄球菌生物膜形成的影响。方法采用分光光度计法检测金黄色葡萄球菌的生长曲线,菌落计数法分析金黄色葡萄球菌的粘附能力,96孔板结晶紫染色法检测金黄色葡萄球菌生物膜的形成。结果不同浓度DNase I对金黄色葡萄球菌的生长无影响,然而能显著抑制细菌生物膜的形成,并呈浓度依赖性。此外,DNase I能抑制不同生长期金黄色葡萄球菌的粘附性。与抗菌药物和DNase I单独应用相比,DNase I和抗菌药物联合应用更能显著抑制和分解金黄色葡萄球菌成熟生物膜。结论 DNase I可以有效抑制金黄色葡萄球菌生物膜的形成,并能增强抗菌药物对金黄色葡萄球菌生物膜的抑制作用。Objective To study the effect of DNase I on biofilm formation of Staphylococcus aureus. Methods The growth curve of S. aureus was detected using a spectrophotometer. The adhesion of S. aureus was analyzed using flat colony counting method,and the biofilm formation was assayed using the 96- well crystal violet staining method. Results Exposure to different concentrations of DNase I did not obviously affect the growth of S. aureus but significantly inhibit the formation of bacterial biofilms in a dose-dependent manner. DNase I inhibited the adhesion of S. aureus at different growth stages. When combined with antibiotics, DNase I resulted in a significant decrease in the established biofilm biomass compared to antibiotics or DNase I used alone. Conclusion DNase I can effectively inhibit biofilm formation of S. aureus and enhance the inhibitory effect of antibiotics against S. aureus biofilms.

关 键 词：金黄色葡萄球菌 DNASE I 生物膜 粘附 抑制作用 

分 类 号：R378.1[医药卫生—病原生物学]