HPCE法同时测定黄连中四种生物碱含量  

Simultaneous Determination of Four Alkaloids Components in Coptidis rhizoma by HPCE

作　　者：翟迎春 金欣[2] 秦亚东[2] 吉玉兰[2] 

机构地区：[1]安徽芜湖县中医医院,安徽芜湖241100 [2]安徽中医药高等专科学校,安徽芜湖241002

出　　处：《现代中药研究与实践》2015年第5期24-26,共3页Research and Practice on Chinese Medicines

摘　　要：目的建立同时测定黄连药材中4种主要生物碱含量的方法。方法建立高效毛细管电泳法,同时测定小檗碱、药根碱、巴马汀碱、黄连碱的含量。结果小檗碱、药根碱、巴马汀碱、黄连碱在10min内完全分离,分别在0.035 5~0.71、0.067 5~0.27、0.045 0~0.180、0.070 0~0.280 mg·ml-1范围内呈现良好的线性关系。结论高效毛细管电泳法可用于测定黄连中主要生物碱的含量。Objective To simultaneously quantify four major alkaloids constituents in Coptidis rhizoma. Methods High Performance Capillary Electrophoresis method was developed to simultaneously quantify Berberine、Jatrorrhizine、Palmatine and Epiberberine in Coptidis rhizoma. Results The Berberine、Jatrorrhizine、Palmatine and Epiberberine were completely separated within 10 minutes,showed good linear relationship in the range of 0.035 5 ~ 0.710、0.067 5 ~ 0.270、0.045 0 ~ 0.180、0.070 0 ~ 0.280 mg·ml-1, respectively. Conclusion HPCE was suitable for the determination of Berberine、Jatrorrhizine、Palmatine and Epiberberine in Coptidis rhizoma.

关 键 词：黄连 生物碱 含量测定 高效毛细管电泳法 

分 类 号：R284.1[医药卫生—中药学]