应用JAR细胞株建立SCID beige小鼠绒毛膜癌移植瘤病理模型  

Establishment of a SCID beige mouse model bearing transplanted human choriocarcinoma using JAR cell line

作　　者：田泉[1] 郑伟[1] 孙戎[1] 薛艳[1] 季伟[1] 安瑞芳[1] 

机构地区：[1]西安交通大学医学院第一附属医院妇产科,陕西西安710061

出　　处：《南方医科大学学报》2015年第10期1406-1410,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81172489)~~

摘　　要：目的建立稳定有效的绒毛膜癌SCID beige小鼠移植瘤模型,观察模型的病程特点和生物学行为。方法将3-5周龄SCID beige雌性小鼠随机分为实验组和对照组;实验组12只小鼠分为A（皮下移植瘤模型）/B（肺转移瘤模型）两组,分别皮下注射或尾静脉注射JAR细胞5×10^6/只,应用形态学、放射性免疫分析法测定β-HCG、小动物活体成像系统（IVIS）和组织学HE染色方法明确JAR细胞皮下移植瘤及肺转移瘤形成等情况。结果实验组接种28 d后,A组小鼠皮下形成质硬肿瘤结节,HE染色符合绒毛膜癌细胞形态学表现;B组小鼠应用IVIS检测显示体内出现单个或多个肿瘤实体包块,HE染色表明肺组织均有不同程度的瘤细胞浸润。接种第14天,实验组β-HCG水平显著高于对照组（P〈0.05）;其中B组β-HCG水平明显高于A组（P〈0.05）。结论 SCID beige小鼠皮下/尾静脉注射JAR细胞可成功构建人绒毛膜癌移植瘤病理模型,该模型能模拟临床绒毛膜癌上皮性实体瘤特性及肺转移特点,是研究人类绒毛膜癌发病机理及实验治疗的良好模型。Objective To establish a mouse model bearing human choriocarcinoma xenograft in severe combined immunodeficient（SCID） beige mice and investigate the disease course and biological behaviors of the tumors. Methods Human choriocarcinoma JAR cells were injected in female SCID beige mice（3-5 weeks old） either subcutaneously（group A, n=6） or via the tail vein（group B, n=6）. Morphological studies, radioactive immunoassay, in vivo tumor imaging and histopathological examinations were performed to confirm JAR cell engraftment at the subcutaneous injection site and in the lungs of the mice.Results On day 28 after tumor cell inoculation, the mice in group A showed palpable subcutaneous nodules, and HE staining revealed morphological features of the nodules consistent with choriocarcinoma cells; in vivo imaging in group B showed single or multiple solid tumor masses in the lungs, and tissue biopsy examination demonstrated varying degrees of tumor cell infiltration. Compared with the control mice, peripheral blood β-HCG levels in both groups A and B increased significantly on day 14 after cell inoculation（P〈0.05）, and the increment was more conspicuous in group B（P〈0.05）. Conclusion Mouse models bearing human choriocarcinoma xenograft can be successfully established by injecting JAR cells either subcutaneously or via the tail vein to mimic the characteristics of epithelial solid tumors and lung metastasis of human choriocarcinoma.

关 键 词：绒毛膜癌 SCID beige小鼠 JAR细胞 移植瘤模型 活体成像系统 

分 类 号：R737.33[医药卫生—肿瘤] R-332[医药卫生—临床医学]