21基因检测对乳腺癌预测和预后的研究  

Prediction of breast cancer and prognosis by 21-gene oncotype Dx

作　　者：鲁凯[1] 刘燕文[1] 李惠[1,2] 刘东[1] 叶红玲[1] 徐良[1] 刘苏[1] 

机构地区：[1]连云港市第二人民医院,222023 [2]江苏省中医院,210029

出　　处：《中华内分泌外科杂志》2015年第5期376-380,共5页Chinese Journal of Endocrine Surgery

基　　金：连云港市科技局社会发展计划（SH1333）

摘　　要：目的：探讨21基因检测对乳腺癌预测和预后的临床意义。方法应用实时定量荧光PCR （ RT-QPCR）技术对100例乳腺癌石蜡包埋组织的增殖、侵袭、人表皮生长因子受体2（ human epidermal growth factor receptor-2，HER2）、激素等相关2l基因定量检测并进行复发评分（recurrence score，RS）。结果100例RS低评分52例、中评分22例、高评分26例。5年复发、转移率分别是1.92％、4.55％和15.38％。21基因评分与发病年龄、肿瘤、组织学分级、淋巴结转移情况无关，与HER2表达密切相关，与ER、PR蛋白表达状态也无显著相关性。结论21基因检测对乳腺癌预测和预后有很好的指导意义。Objective To study the prognostic and predictive significance of 21-gene assay （ oncotype DX）in breast cancer.Methods Real-time quantitative PCR（RT-QPCR）was used to detect 21 gene expression in breast cancer tissues （100 cases）and recurrence score（RS）was calculated.Results Among the 100 cases, 52 cases had low RS , 22 cases had middle RS , and 26 cases had high RS .The recurrence rate of five years was 1.92%,4.55%and 15.38%respectively.21 gene expression had nothing to do with patients＇age, tumor size, histological grade , lymph node metastasis state , ER expression , or PR expression .It was associated with HER 2 expression .Conclusions 21 genes is a good prediction factor in breast cancer and its prognosis .

关 键 词：乳腺癌 21基因 荧光实时定量PCR 预后 

分 类 号：R737.9[医药卫生—肿瘤]