VEGF-D、D2-40和 MMP-7表达及其与甲状腺乳头状癌淋巴结转移相关性研究  

Expression of VEGF-D, D2-40, and MMP-7 and its relationship with cervical lymph node metastasis in thyroid papillary carcinoma

作　　者：程维刚[1] 郭万里[2] 刘九洲[1] 殷德涛[3] 

机构地区：[1]郑州大学附属洛阳中心医院普外科3病区,471009 [2]郑州大学附属洛阳中心医院肿瘤病区,471009 [3]郑州大学第一附属医院甲状腺外科、河南省高等学校临床医学重点学科开放实验室,450052

出　　处：《中华内分泌外科杂志》2015年第5期401-404,408,共5页Chinese Journal of Endocrine Surgery

基　　金：国家自然科学基金（81372863）;郑州市科技领军人才项目（131PLJRC676）

摘　　要：目的：探讨甲状腺乳头状癌（ papillary thyroid carcinoma ，PTC）组织中血管内皮生长因子（vascular endothelial growth factor-D，VEGF-D）、D2-40及基质金属蛋白酶-7（matrix metalloproteinase-7， MMP-7）的表达水平及其与PTC的淋巴管密度、分布和颈部淋巴结转移之间的关系。方法采用免疫组化SP法检测PTC伴有淋巴结转移癌组织、PTC未伴有转移癌组织和正常甲状腺组织中VEGF-D、D2-40和MMP-7的表达水平，并对其微淋巴管密度（micro lymphatic density，MLD）进行计数。结果 VEGF-D在转移PTC及正常甲状腺组织中的表达差异具有统计学意义（χ2＝13.074，P＝0.000，P＜0.0167）；D2-40在转移PTC边缘区及未转移PTC边缘区的表达差异具有统计学意义（P＜0.05）；MMP-7在转移PTC及未转移PTC表达差异具有统计学意义（91.67％vs 73.33％，P＜0.05）；MLD在转移PTC、未转移PTC和正常甲状腺组织中的差异具有统计学意义（11.7±3.5、8.9±3.1、3.9±2.7，P＜0.05）。结论 VEGF-D、D2-40和MMP-7在PTC组织中高表达且可能参与PTC颈部淋巴结的转移。Objective To detect the expression levels of vascular endothelial growth factor D （ VEGF-D） , D2-40 and MMP-7 in papillary thyroid carcinoma （ PTC） and to explore their relationship with micro lymphat-ic density, distribution,and cervical lymph node metastasis .Methods SP immunohistochemistry was used to study the expression of VEGF-D, D2-40 and MMP-7 in 38 cases of PTC with cervical lymph node metastasis , 45 cases of PTC without cervical lymph node metastasis , and 22 cases of normal thyroid tissues .Micro lymphatic density （ MLD） was counted .Results The expression level of VEGF-D in PTC with cervical lymph node metasta-sis and normal thyroid tissues had significant difference （χ2 =13.074, P=0.000, P〈0.0167）.The expression level of D2-40 in PTC with and without cervical lymph node metastasis had significant difference （ P〈0.05 ） . MMP-7 expression in PTC with and without cervical lymph node metastasis had statistical significance （ 91.67%vs 73.33%, P〈0.05）.MLD in PTC with and without cervical lymph node metastasis and in normal thyroid tis-sues had statistical difference （11.7 ±3.5 vs 8.9 ±3.1 vs 3.9 ±2.7, P〈0.05）.Conclusion VEGF-D, D2-40 and MMP-7 are highly expressed in PTC and they are possibly related with cervical lymph node metastasis of PTC.

关 键 词：甲状腺乳头状癌 VEGF—D D240 MMP-7 

分 类 号：R736.1[医药卫生—肿瘤]