PGCsiRNA-AQP1重组质粒抗喉癌生长的体内研究  

The inhibiting role of recombinant plasmid PGCsi-AQP1on laryngeal carcinoma in vivo

作　　者：关桂梅[1] 朱冬冬[1] 董震[1] 沙骥超[1] 

机构地区：[1]吉林大学中日联谊医院耳鼻咽喉科,长春130033

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第21期1886-1889,1893,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:以水通道蛋白(AQP1)为靶点构建中性脂质体1,2-二油酰基磷脂酰胆碱(DOPC)介导的PGCsiRNA-AQP1重组质粒,转染至喉癌荷瘤小鼠体内,诱导RNA干扰(RNAi)沉默AQP1表达,探讨其对喉癌生长的抑制作用。方法:建立喉癌BALB/c小鼠移植模型,随机分为尾静脉及瘤周注射组、阴性及空白对照组(n=5),重组质粒转入小鼠瘤体,观察肿瘤细胞核固缩及凋亡,计算瘤体积及抑瘤率;免疫组织化学法检测瘤体内AQP1表达和微血管密度。结果:动物模型建立成功。1苏木精-伊红染色示尾静脉和瘤周注射组可见核固缩及凋亡小体,出现大量凋亡细胞;22个注射组的瘤体体积均小于对照组(P<0.01);尾静脉和瘤周注射组抑瘤率分别为52.4%和53.5%(P>0.05);3免疫组织化学法示2个注射组AQP1阳性细胞表达率和微血管密度均小于对照组(P<0.01)。结论:由DOPC介导能够将PGCsiRNA-AQP1重组质粒转入BALB/c小鼠瘤体内,并有效诱导RNAi,抑制小鼠体内喉癌组织中的AQP1及微血管生成,从而抑制喉癌的生长和发展。Objective：To construct a kind of recombinant plasmid PGCsi-AQP1 delivery with DOPC and explore the inhibit effect of laryngeal carcinoma by RNAi targeting AQP1 in vivo. Method： Male BALB/c mice, 6 weeks of age transplanted with laryngeal carcinoma cell line Hep-2, four groups were divided randomly.. Tail vein injection group （TVIG）, Carcinoma around injection group （CAIG）, negative control group （NCG） and blank control group （BCG）. The recombinant plasmid PGCsi-AQP1 delivery with DOPC were inject into tail vein or sur- rounding tumor. HE pathological slides and tumor size were observed and inhibitory rate was figured up. The lev- el of AQP1 protein expression and high microvessel density were detected by Immunohistochemical staining （IHC）. Result：We constructed BALB/c mice models of laryngeal carcinoma successfully （1） HE staining., cell putrescence, nuclear pyknosis and apoptotic bodies were more in the tumor tissues of experimental groups than two control groups. （2）The total volumes of tumor in experimental group were both smaller than in two control groups （P〈0.01）. The inhibition rate of TVIG and CAIG were 52.4% and 53.5% respectively and there was no significant difference （P〈0.05）. （3）IHC.. the AQP1 positive cells and microvessel density in TVIG and CA1G were both less than in two control groups （P〈0.01）. Conclusion： Neutral lipsomes DOPC could help carriaging the recombinant plasmid PGCsi-AQP1 to tumor and then play an inhibit role in laryngeal carcinoma tissue by RNAi targeting AQP1 in vivo.

关 键 词：喉癌 水通道蛋白 RNA干扰 重组质粒 中性脂质体 

分 类 号：R739.6[医药卫生—肿瘤]