正交设计优化石韦多糖的纤维素酶法提取工艺  

Optimization of Cellulase Extraction Technology of Polysaccharides from Folium Pyrrosiae by Orthogonal Test

作　　者：黄莹[1] 李芸达[1] 程世嘉 黄挺章 李远辉[1] 郭圣奇 黄锁义[2] 

机构地区：[1]右江民族医学院临床学院,广西百色533000 [2]右江民族医学院药学院,广西百色533000

出　　处：《化学世界》2015年第10期592-596,共5页Chemical World

基　　金：国家中医药管理局"十二五"中医药重点学科中药化学建设项目(国中医药人教发[2012]32号);广西重点学科药物化学建设项目(桂教科研[2013]16号);广西高校科技创新能力提升工程建设项目(桂教科研〔2014〕14号)

摘　　要：采用单因素试验和正交试验,以石韦多糖得率为指标,研究pH值、酶用量、酶解温度和时间对石韦多糖提取效果的影响。优选纤维素酶法提取石韦多糖的工艺条件。正交试验结果表明,影响石韦多糖得率的因素次序:酶解温度>酶解pH值>酶解时间>酶用量。得出纤维素酶法提取的最佳工艺条件为:酶解pH6.0,酶用量为8.0mL,酶解温度50℃,酶解时间50min。在优化的条件下进行提取实验,石韦多糖得率为6.58%。说明纤维素酶法提取石韦多糖省时高效,可为石韦多糖的大规模生产提供可借鉴的新方法。To optimize the cellulase extraction technology,the influence of enzymolysis pH,enzyme dosage,enzymolysis temperature and time on yield of polysaccharide fromFolium Pyrrosiae was studied with the single factor experiment and the orthogonal experiment.The orthogonal experiments showed that the influencing factors were in the sequence of enzymolysis temperature〉enzymolysis pH〉enzymolysis time〉enzyme dosage.The results showed that the optimum conditions leading to 6.58% polysaccharide yield were as follows：cellulase of 8.0mL,hydrolysis for 50 min,pH of 6.0and 50℃.The extraction of polysaccharide fromFolium Pyrrosiae by cellulose enzyme is a simple,quick and efficient method,which can be used for industrial production of polysaccharide.

关 键 词：石韦 多糖 纤维素酶 正交设计 

分 类 号：R284[医药卫生—中药学]