结肠电刺激对肠易激大鼠模型内脏高敏感性的影响  

Effect of Colonic Electrical Stimulation on Visceral Hypersensitivity in Rats with Irritable Bowel Syndrome

作　　者：覃晓日 谭琰[2] 孙晓宁[3] 

机构地区：[1]南华大学,湖南衡阳421001 [2]海南医学院附属医院消化内科 [3]海南省人民医院消化内科

出　　处：《中南医学科学杂志》2015年第5期497-501,共5页Medical Science Journal of Central South China

基　　金：海南省重点科技计划项目(SQ2013ZDXM0385)

摘　　要：目的探讨结肠电刺激对应激诱导产生的内脏高敏感性的影响。方法 48只大鼠等分为模型组、对照组,模型组由双重应激诱导产生,其内脏高敏感性程度由肌电图及腹部撤退反射的分数来评估,对照组不接受双重应激。建模后在所有大鼠的结肠浆膜层包埋刺激电极,待手术恢复后分别对各组大鼠进行长脉冲电刺激、短串脉冲电刺激、无电刺激,刺激结束后再次对模型组、对照组大鼠的内脏高敏感性程度进行评估。结果通过应激诱导,模型组大鼠对结肠扩张压力的敏感性与对照组比较有显著增加(P<0.01)。串短脉冲、长脉冲结肠电刺激相较无电刺激能够显著降低大鼠的内脏高敏感性(P<0.01)。结论串短脉冲、长脉冲结肠电刺激能够显著降低大鼠内脏高敏感性。Objective To investigate whether stress-induced visceral hypersensitivity could be alleviated by colonic electrical stimulation（ CES）. Methods 48 male rats were divided into model group and control group,visceral hypersensitivity model was induced by a 6 d heterotypic intermittent stress,the extent of visceral hypersensitivity was quantified by electromyography and abdominal withdrawal reflx scores. After the modeling,all rats were equipped with electrodes in descending colon. After recovering from surgery,CES with long pulses,CES with trains of short pulses,sham CES was performed. Finally,the extent of visceral hypersensitivity will be quantified again in model group. Results Rats displayed an increased sensitivity to colorectal distention,after a 6 d heterotypic intermittent stress compared to control group at different distention pressures（ P 0. 01）. CES with trains of short pulses and long pulses significantly attenuated the hypersensitive responses to colorectal distention compared with sham CES group（ P 0. 01）. Conclusion CES with long pulses,CES with trains of short pulses all can significantly alleviate stress-induced visceral hypersensitivity.

关 键 词：结肠电刺激 肠易激综合征 内脏高敏感性 肌电图 

分 类 号：R574.6[医药卫生—消化系统]