P16、Ki-67在鳞状上皮病变宫颈组织中的表达及其与HPV联合检测的意义  

The expression of P16,Ki-67 in cervical squamous epithelial lesions and the significance of their detection combined with HPV

作　　者：潘燕珍 丁红梅 

机构地区：[1]云浮市人民医院妇产科,广东云浮527300

出　　处：《国际检验医学杂志》2015年第20期2942-2944,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨P67、Ki-67在鳞状上皮病变宫颈组织中的表达及其与人乳头瘤病毒（HPV）联合检测的意义。方法收集手术切除的宫颈组织188例。其中,宫颈癌48例（宫颈癌组）,宫颈上皮内瘤变（CIN）100例（CIN组）,慢性宫颈炎40例（宫颈炎组）。应用免疫组化检测宫颈组织中P16、Ki-67的表达情况,采用PCR技术检测宫颈组织中HPV感染情况。结果宫颈癌组、CIN组P16、Ki-67表达阳性率均高于宫颈炎组（P〈0.05）,宫颈癌组P16、Ki-67表达阳性率均高于CINⅠ和CINⅡ组（P〈0.05）,但宫颈癌组与CINⅢ组间比较差异均无统计学意义（P〉0.05）。随着CIN级别的增高,P16、Ki-67表达阳性率增高,CINⅠ～Ⅲ3组间比较差异均有统计学意义（P〈0.05）。宫颈癌组、CINⅢ组P16、Ki-67过表达率均高于其他3组（P〈0.05）;CINⅠ～Ⅲ3组中P16和Ki-67过表达率比较,差异均有统计学意义（P〈0.05）。P16表达强度与HPV阳性率呈正相关（rs=0.706,P=0.011）;Ki-67表达强度与HPV阳性率呈正相关（rs=0.695,P=0.021）。结论可以通过联合检测HPV、P16、Ki-67对CIN、早期宫颈癌进行辅助诊断,并对CIN进展进行预测。Objective To study the expression of P16 ,Ki‐67 in cervical squamous epithelial lesions and the significance of their detection combined with human papillomavirus（HPV） .Methods 188 cases of cervical tissues from surgical excision were collected , including 48 cases of cervical cancer ,100 cases of cervical intraepithelial neoplasia（CIN） ,and 40 cases of chronic cervicitis ,which were set as cervical cancer group ,CIN group and cervicitis group ,respectively .Expression of P16 ,Ki‐67 in cervical tissue were de‐tected by using immunohistochemistry ,and the HPV infection were detected by using PCR technology .Results The positive ex‐pression rates of P16 and Ki‐67 in cervical cancer ,CIN group was significantly higher than those in cervicitis group（P〈0 .05） ,and the positive expression rates of P16 and Ki‐67 in cervical cancer group were higher than those in CINⅠ and CINⅡ group （P〈0 . 05） ,but between the cervical cancer group and CIN Ⅲ group there were no statistically significant difference（P〉0 .05） .With the increase of CIN level ,the positive expression rates of P16 and Ki‐67 increased ,among the three CIN groups there were significant difference（P〈0 .05） .In cervical cancer group and CINⅢ group ,the expression rates of P16 and Ki‐67 were both significantly high‐er than the other 3 groups（P〈0 .05） .In CINⅠ - Ⅲ groups ,the over expression rates of P16 and Ki‐67 were statistically different （P〈0 .05） .The expression of P16 positively correlated with the positive rates of HPV infection （rs =0 .706 ,P=0 .011）;the ex‐pression of Ki‐67 positively correlated with the positive rates of HPV infection（rs=0 .695 ,P=0 .021） .Conclusion CIN and cervi‐cal cancer of early stage could be diagnosed and the pathological progress of CIN could be predicted by using the combined detection of P16 ,Ki‐67 and HPV .

关 键 词：宫颈上皮内瘤变 宫颈癌 KI-67基因 P16基因 人乳头瘤病毒 

分 类 号：R737.33[医药卫生—肿瘤]