XRCC1基因在白血病中表达的研究  

The study on the expression of XRCC1 in chronic myelocytic leukemia

作　　者：柴晓静[1] 李娟[1] 陆莉[1] 

机构地区：[1]兰州大学第一医院中心实验室,甘肃兰州730000

出　　处：《国际检验医学杂志》2015年第20期3005-3006,共2页International Journal of Laboratory Medicine

基　　金：兰州大学中央高校基本科研业务费专项资金资助项目(lzujbky-2012-192);甘肃省青年科技研究基金项目资助项目(1308RJYA073)

摘　　要：目的通过比较白血病患者与健康人群人类X射线交叉互补修复基因1(XRCC1)基因多态性和超甲基化水平,探讨XRCC1与白血病的相关性。方法利用限制性片段长度多态性聚合酶链反应(PCR-RFLP)和甲基化特异聚合酶联反应(MSP)的方法检测150例白血病患者(病例组)和150例健康体检者(对照组)XRCC1基因多态性和启动子区超甲基化水平。结果在各型白血病中XRCC1基因rs1799782、rs25487、rs25489位点基因型有不同程度的变化。病例组各亚组的XRCC1基因超甲基化阳性率与对照组比较,差异均无统计学意义(P>0.05)。结论多态性位点基因型分布及等位基因频率和白血病易感有一定的相关性,但XRCC1基因在白血病中可能不存在超甲基化现象。Objective To investigate whether XRCC1 was associated with the occurrence of leukemia through the comparison of XRCC1 gene′s polymorphism and hypermethylation between leukemia patients and healthy people .Methods Restriction fragment length polymorphism polymerase chain reaction （PCR‐RFLP） and methylation specific polymerase chain reaction（MSP） were used to detect the polymorphism and promoter region′s methylation of XRCC1 gene in 150 patients with leukemia（patients group） and 150 healthy persons （control group） .Results In different types of leukemia patients ,the genotype of XRCC1 gene loci rs1799782 , rs25487 and rs25489 loci changed in different degrees .The positive rate of XRCC1 gene methylation in different subgroups of pa‐tients group were not statistically significantly different from that of control group（P〉0 .05） .Conclusion Genotype distribution and allele frequency and leukemia susceptible have some correlation ,but hypermethylation phenomenon may not exist in XRCC1 gene in leukemia .

关 键 词：人类X射线交叉互补修复基因1 白血病 基因多态性 甲基化 

分 类 号：R733.7[医药卫生—肿瘤]