红提葡萄V_C含量的可见/近红外检测模型  

Determination of vitamin C in red globe grape based on visible/near-infrared diffuse reflectance spectroscopy

作　　者：陈辰[1,2] 鲁晓翔[1,2] 张鹏[3,4] 陈绍慧[3,4] 李江阔[3,4] 

机构地区：[1]天津商业大学生物技术与食品科学学院,天津300134 [2]天津市食品生物技术重点实验室,天津300134 [3]国家农产品保鲜工程技术研究中心,天津300134 [4]天津市农产品采后生理与贮藏保鲜重点实验室,天津300384

出　　处：《食品与机械》2015年第5期70-74,共5页Food and Machinery

基　　金："十二五"国家科技支撑计划项目(编号:2012BAD38B01);天津市高等学校创新团队培养计划项目(编号:TD12-5049)

摘　　要：为了建立红提葡萄VC含量的可见/近红外漫反射光谱检测模型,并评价其应用价值,应用不同的化学计量学建模方法和光谱预处理方法,在不同特征波长区间内建立定标模型,讨论建模效果,并通过预测集样品验证最优模型的精度。结果显示:在全光谱范围内,应用改进偏最小二乘法(MPLS)结合一阶导数、5点平滑、加权多元离散校正(WMSC)处理得到的定标模型效果最优,交互验证标准差SECV为0.054 3,定标决定系数R2cv为0.920 2,预测决定系数R2p为0.931 8,预测标准差SEP为0.050 0,残差平方和PRESS为0.188 0,预测相对分析误差RPD为3.640 0。故应用可见/近红外漫反射技术对红提葡萄果实VC含量进行快速无损检测是可行的,模型稳定且精度较高。For establishing the determination model of vitamin C content of Red Globe Grape using visible/near infrared diffuse reflectance spectroscopy,and evaluating its application value,effects on scaling model results of different chemometry modeling methods,different spectra pretreatment methods and different effective wavelength intervals were discussed,and the samples in prediction set were used to verified the accuracy of the optimal model.The results showed that in the whole spectral range,application of modified partial least squares（MPLS）combined with first derivative,5points smoothing,weighted multivariate discrete correction（WMSC）could obtain the optimal calibration models,the cross validation error（SECV）was 0.054 3,the calibration determination coefficient（R2cv）was 0.920 2,the prediction determination coefficient（R2p）was0.931 8and the standard error of prediction（SEP）was 0.050 0,the sum of squared residuals（PRESS）was 0.188 0,the relative analysis of the forecast error（RPD）was 3.640 0.Therefore,applying visible/near infrared diffuse reflectance technique to quick and nondestructive detection of vitamin C in Red Globe grape is feasible,the model is stable and with high precision.

关 键 词：可见/近红外漫反射光谱 红提葡萄 VC 检测模型 

分 类 号：O657.3[理学—分析化学] TS255.7[理学—化学]