杜香多糖的抗氧化活性及物理性质研究  

Antioxidative activity and physical property of polysaccharide from Ledum palustre L.

作　　者：张乔会[1,2,3] 王建中[2,3] 逄锦慧 崔洁[2,3] 杨喆[2,3] 罗兴武[1] 

机构地区：[1]湖北民族学院科技学院,湖北恩施445000 [2]北京林业大学生物科学与技术学院,北京100083 [3]林业食品加工与安全北京市重点实验室,北京100083

出　　处：《食品与机械》2015年第5期206-209,共4页Food and Machinery

基　　金：林业公益性行业科研专项(编号:201004081)

摘　　要：以杜香为原料,采取水提醇沉法提取杜香多糖,利用红外和紫外分析,发现提取物含多糖典型基团,粗提物中多糖含量为69.95%。通过测定多糖对DPPH自由基（DPPH·）、超氧阴离子自由基（·O-2）、羟基自由基（·OH）的清除能力、总还原能力,得到杜香多糖清除DPPH·、·O-2、·OH的EC50值分别为0.52,1.41,1.71mg/mL,还原力接近BHT且与浓度呈正相关,杜香多糖具有较强的抗氧化活性。杜香多糖在190℃以内稳定,在190~550℃范围内发生热解,其结晶度为3.6%,结晶度低,分子刚性差。The polysaccharide was extracted from Ledum,and the content and characteristics of polysaccharide fromLedum were investigated by IR,UV spectra,thermal analysis（TG）and X-ray diffraction（XRD）.The results from IR and UV spectra showed that the extracts containing the typical group of polysaccharide.It was noted that the content of Ledumpolysaccharide was 69.95%.In addition,the antioxidant activity containing total reductive ability,scavenging ability for DPPH radical（DPPH·）,hydroxyl radical（·OH）and superoxide anion radical（· O-2）were investigated.The results showed that the EC50 for scavenging DPPH·,·O-2,·OH were0.52,1.41,1.71 mg/mL,which means that the polysaccharide fromLedumpossessed a notable antioxidant activity.Moreover,the antioxidant ability was close to BHT and increased with the growth of the content of polysaccharide.The results of thermal stability from thermal analysis（TG）showed that the chemical decomposition of polysaccharide fromLedum was occurred at 190 ℃.The crystallinity of polysaccharide from Ledum was 3.6%,the low degree of crystallinity resulted in a poor molecular rigidity.

关 键 词：杜香 多糖 抗氧化 物理性质 X射线衍射 热重分析 

分 类 号：TQ281[化学工程—有机化工]