miRNA-411对乳癌细胞侵袭和迁移的影响  

EFFECT OF MIRNA-411ON INVASION AND MIGRATION OF HUMAN BREAST CANCER CELLS

作　　者：房建凯[1] 陈枉枉 郭玲玲[1] 

机构地区：[1]苏州大学医学部病理学与病理生理学教研室,江苏苏州215213 [2]苏州大学医学部解剖学教研室暨细胞神经生物学研究室,江苏苏州215213

出　　处：《齐鲁医学杂志》2015年第6期637-640,共4页Medical Journal of Qilu

摘　　要：目的探讨微小RNA-411(miRNA-411)对人乳癌细胞株MDA-MB-231增殖、侵袭和迁移的影响。方法采用实时定量PCR方法检测高转移性乳癌细胞MDA-MB-231与低转移性乳癌细胞MCF-7中miRNA-411的表达。采用LipofectamineTM2000将miRNA-411成熟子(Mimic)转染至MDA-MB-231细胞中,通过采用实时定量PCR方法检测miRNA-411 Mimic的转染效率,Transwell法检测细胞的迁移和侵袭能力,免疫蛋白印迹法检测E-钙黏素(E-cadherin)和波形蛋白(Vimentin)的表达,MTT实验检测MDA-MB-231细胞的增殖能力。结果 miRNA-411在MDA-MB-231细胞中的表达水平显著低于MCF-7细胞(t=7.45,P<0.01)。miRNA-411Mimic瞬时转染MDA-MB-231细胞后,其迁移和侵袭能力均受到明显的抑制(t=7.24、6.62,P<0.01),而且经转染的细胞E-cadherin表达升高,而Vimentin表达下降。然而,miRNA-411Mimic对MDA-MB-231细胞增殖能力无明显影响(P>0.05)。结论 miRNA-411在高转移性乳癌细胞中低表达,转染miRNA-411 Mimic后乳癌细胞MDAMB-231迁移和侵袭能力明显减弱,其迁移和侵袭能力的下降并非通过降低MDA-MB-231细胞的增殖能力所致,而是可能通过逆转MDA-MB-231细胞的上皮-间质转化过程实现的。Objective To investigate the impact of microRNA-411（miRNA-411）on proliferation,migration and invasion of human breast cancer cell line MDA-MB-231. Methods The expression of miRNA-411 was detected in highly metastatic breast cancer MDA-MB-231 cells and lowly metastatic breast cancer MCF-7cells by real-time PCR assay.Employing LipofectamineTM2000,miRNA-411 mimic was transfected into breast cancer MDA-MB-231 cells.The transfection efficiency of miRNA-411 mimic was detected by real-time PCR assay,and the migration and ability of the cells measured by Transwell assay,the expressions of E-cadherin and vimentin by Western blotting,and the proliferation of the cells by MTT assay. Results The expression of miRNA-411 in MDA-MB-231 cells was significantly lower than MCF-7cells（t=7.45,P〈0.01）.After miRNA-411 mimic was transiently transfected into MDA-MB-231 cells,the migration and invasion of MDA-MB-231 cells were significantly inhibited（t=7.24,6.62;P〈0.01）.The expression of E-cadherin in infected cells elevated,and that of Vimentin declined.No significant impact of miRNA-411 mimic on the reproductive activity of MDA-MB-231 cells was noted（P〉0.05）. Conclusion miRNA-411 is lowly expressed in highly metastatic breast cancer cells.The migration and invasion of breast cancer MDA-MB-231 cells are significantly inhibited after transfection with miRNA-411 mimic,the suppression of the migration and invasion is not due to reducing the proliferation ability of MDA-MB-231 cells,but possibly through inversion of epithelial-mesenchymal transition of MDA-MB-231 cells.

关 键 词：乳房肿瘤 MDA-MB-231 miRNA-411 细胞运动 肿瘤侵润 

分 类 号：R737.9[医药卫生—肿瘤]