SynphiLin-1 siRNA对6-OHDA诱导帕金森病大鼠黑质区NF-κB表达影响  

THE EFFECT OF SYNPHILIN-1SIRNA ON THE EXPRESSION OF NF-κB IN SUBSTANTIA NIGRA OF PARKINSON DISEASE RAT MODEL INDUCED BY 6-HYDROXYDOPAMINE

作　　者：张永军[1] 薛莉[2] 魏梅[3] 谢安木[1] 

机构地区：[1]青岛大学附属医院神经内科,山东青岛266003 [2]青岛大学附属医院康复科,山东青岛266003 [3]青岛大学附属医院内分泌科,山东青岛266003

出　　处：《齐鲁医学杂志》2015年第6期658-660,共3页Medical Journal of Qilu

基　　金：山东省自然科学基金资助项目(ZR2012CM028)

摘　　要：目的研究SynphiLin-1siRNA对6-羟基多巴胺（6-OHDA）诱导的大鼠黑质区核转录因子（NF-κB）表达的影响,探讨SynphiLin-1及NF-κB在帕金森病（PD）炎性发病机制中的作用。方法选择Wistar大鼠36只,随机分成4组,每组9只,分别向大鼠左侧黑质致密部注入6-OHDA、SynphiLin-1siRNA、抗坏血酸生理盐水及siRNA作为PD组、siRNA组、阳性对照组、siRNA对照组。采用免疫组织化学法检测各组大鼠黑质区酪氨酸羟化酶（TH）、SynphiLin-1及NF-κB表达,并进行比较。结果与阳性对照组比较,PD组大鼠黑质区SynphiLin-1、NF-κB阳性细胞显著增加,TH阳性细胞数下降;与PD组比较,siRNA组大鼠黑质区NF-κB、SynphiLin-1阳性细胞明显减少,TH阳性细胞数显著增加,差异均有显著性（F=8.6~14.8,P〈0.05）。siRNA组TH阳性细胞数与阳性对照组比较差异无统计学意义（P〉0.05）。结论 SynphiLin-1可能是6-OHDA诱导的PD大鼠中脑黑质区炎症反应重要上游调节蛋白,与NF-κB相互作用,参与PD发病的神经炎性过程。Objective To investigate the effect of SynphiLin-1on expression of nuclear factor kappa B（NF-κB）in substantia nigra（SN）in rats induced by 6-Hydroxydopamine（6-OHDA）and explore SynphiLin-1and NF-κB in the inflammatory pathogenesis of Parkinson’s disease（PD）. Methods Thirty-six Wistar rats were equally randomized to four groups and injected into left substantia nigra（SN）with 6-OHDA,SynphiLin-1siRNA,ascorbic plus normal saline and siRNA of rats in PD group,siRNA group,positive control group and siRNA control group,respectively.Immunohistochemical method was used to detect and compare the expressions of tyrosine hydroxylase（TH）,SynphiLin-1and NF-κB in the SN. Results Compared with the positive control group,the number of positive NF-κB and SynphiLin-1cells in the PD group increased,and that of positive TH cells decreased;compared with PD group,the number of positive NF-κB and SynphiLin-1cells in siRNA group decreased,and that of TH cells increased（F=8.6-14.8,P〈0.05）.No statistical difference was noted in the number of positive TH cells between siRNA group and positive control group（P〉0.05）. Conclusion SynphiLin-1might be an important upstream regulatory protein in inflammatory reaction in substantia nigra of midbrain in rat induced by 6-OHDA,which interacts with NF-κB and involves in the pathogenesis of Parkinson’s disease and its process of neuritis.

关 键 词：SYNPHILIN-1 NF-ΚB 羟多巴胺 帕金森病 酪氨酸单氧化酶 

分 类 号：R742.5[医药卫生—神经病学与精神病学]