Eupatilin对黄嘌呤氧化酶活性抑制的实验研究  

Study on Inhibitory Effect of Eupatilin on Xanthine Oxidase Activity

作　　者：王海东[1] 谢建祥[2] 林伟青[1] 

机构地区：[1]江西省人民医院,南昌330006 [2]江西省儿童医院,南昌330006

出　　处：《实用中西医结合临床》2015年第9期87-89,共3页Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:通过体外酶学试验分析Eupatilin对黄嘌呤氧化酶活性的影响及抑制机理。方法:利用紫外分光光度计观测尿酸生成导致的吸光度变化值,测定波长为292 nm,并以黄嘌呤为底物根据测定结果绘制Lineweaver-Burk和Dixon图。结果:Eupatilin对黄嘌呤氧化酶表现很强的抑制作用,其Ki值为0.128 6μg/ml,抑制类型为混合型;而别嘌呤醇作为对照,其Ki值为0.62μg/ml,抑制类型为竞争性抑制。结论:Eupatilin具有明显的黄嘌呤氧化酶抑制活性。Objective： To research the influence and inhibitory mechanism of Eupatilin on the activity of Xanthine Oxidase by vitro enzymatic test. Methods： Determined the absorbance changes caused by the formation of uric acid value by using UV spectrophotometer,the detection wavelength was 292 nm, according to the measured results, draw the Lineweaver Burk and Dixon plots with Xanthine as substrate. Results： Eupatilin had strong inhibition on Xanthine Oxidase, the Ki value was 0.128 6 μg/ml, the type of inhibition is of mixed type; and with Allopurinol as a reference, the Ki value was 0.62 μg/ml, the type of inhibition was competitive inhibition. Conclusion：Eupatilin has the obvious inhibitory activity of Xanthine Oxidase.

关 键 词：异泽兰黄素 黄嘌呤氧化酶 黄嘌呤 活性抑制 

分 类 号：R285.5[医药卫生—中药学]