茶多酚对帕金森病猴多巴胺能神经元保护作用的研究  

Protective effect of tea polyphenols on dopaminergic neuron in Parkinsonism disease monkeys

作　　者：陈宏伟 于兰[2,3] 陈敏[2,3] 李旭冉[2,4] 李昕[2,4] 杨巍巍[2,4] 王鹏[2,4] 于顺[2,4,5] 

机构地区：[1]朝阳区十八里店社区卫生服务中心,北京100122 [2]首都医科大学宣武医院神经生物学研究室,北京100053 [3]桂林医学院解剖学教研室,桂林541004 [4]帕金森病研究北京重点实验室,北京100053 [5]北京脑重大疾病研究院帕金森病研究所,北京100053

出　　处：《首都医科大学学报》2015年第5期689-693,共5页Journal of Capital Medical University

基　　金：国家重点基础研究发展计划(2011CB504101);国家自然科学基金(81071014;81371200;81401042);国家科技支撑计划课题(2012BAI10B03);北京市自然科学基金(7122035);首都卫生发展科研专项课题(2011-4001-01)~~

摘　　要：目的探索茶多酚对1-甲基-4-苯基-1,2,3,6-四氢吡啶（1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP）诱导的帕金森病猴神经元的保护作用及机制。方法健康雌性食蟹猴年龄10~12岁,采用数字表法随机分为4组：正常对照组、茶多酚对照组（TP组）、帕金森病模型组（MPTP组）和茶多酚治疗组（MPTP＋TP组）,每组4只。正常对照组不进行任何处理;茶多酚对照组灌胃给予茶多酚80 d;PD模型组静脉注射MPTP诱导PD模型,茶多酚治疗组在PD模型建立后给予茶多酚治疗80 d,每周2次用帕金森病临床行为评价量表评价各组动物运动损伤。实验动物经安乐死后,分离脑组织,高效液相色谱分析纹状体多巴胺及其代谢产物含量,免疫组织化学和体视学分析黑质多巴胺能神经元变化。结果 TP能减少MPTP诱导的食蟹猴黑质多巴胺能神经元的损伤并缓解其运动障碍。结果首次在灵长类动物体内实验证实茶多酚能有效减少多巴胺能神经元的损伤并改善其运动机能。Objective To investigate the protecting efficacy of tea polyphenols on dopaminergic neurons in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（ MPTP） induced Parkinson＇s disease monkeys and its mechanism. Methods Healthy female cynomolgus monkeys10 to 12 years old were randomly divided into four groups： normal control group（ n = 4）,TP group（ n = 4）,MPTP group（ n = 4）,and MPTP ＋ TP group（ n = 4）. Control group were not given any treatment; The TP group monkeys were treated with TP by gastrogavage once daily for 80 consecutive days. In MPTP and MPTP ＋ TP groups,MPTP was injected intravenously as MPTP-HCl to induce PD model. The MPTP ＋ TP groups were treated with TP by gastrogavage once daily for 80 consecutive days after making PD model. Motor deficits were quantified twice a week by a previously validated Parkinsonian monkey clinical rating scale. All animals were euthanized and the brains tissues were rapidly separated. To measure the levels of dopamine and its metabolites in the striatum were measured using HPLC method. Dopaminergic neurons were identified by immunohistochemistry and stereology. Results Tea polyphenols alleviated MPTPinduced injury of nigral dopaminergic neurons and improved motor impairments in cynomolgus monkeys（ P〈0. 05）. Conclusion This study showed first in vivo evidence that TP could alleviate dopaminergic neuronal injury and motor impairments in nonhuman primates.

关 键 词：茶多酚 运动障碍 多巴胺能神经元 帕金森病 

分 类 号：R338[医药卫生—人体生理学]