胡桃醌脂质体的制备及理化性质  

Development and characterization of Juglone liposomes

作　　者：杨倩[1] 罗国伟[1] 彭海龙[2] 熊华[1] 邱俊明[3] 

机构地区：[1]南昌大学食品学院,江西南昌330031 [2]南昌大学资源环境与化学工程学院,江西南昌330031 [3]南昌大学期刊社,江西南昌330031

出　　处：《南昌大学学报（工科版）》2015年第3期234-239,共6页Journal of Nanchang University(Engineering & Technology)

基　　金：国家自然科学基金资助项目(21201098);江西省青年科学基金资助项目(20142BAB213003;20151BAB203029);江西省教育厅青年基金资助项目(GJJ13039)

摘　　要：采用乙醚注入法制备胡桃醌脂质体,在单因素试验基础上,以膜材比、脂药比、注入速度为影响因素,包封率为响应值,利用Box-Behnken试验设计原理,采用3因素3水平的响应面分析法,对胡桃醌脂质体制备工艺进行优化。胡桃醌脂质体的最佳制备条件为膜材质量比4.86∶1.00,脂药质量比10.92∶1.00,注入速度1.52 m L·min-1,采用最佳条件制备的胡桃醌脂质体包封率较高,验证实验测得胡桃醌脂质体的包封率为79.80%,与模型预测值相对误差为0.033%,重现性好。Juglone liposomes were prepared by ether injection method. One-factor-at-a-time design was used to investigate the effects of five process parameters on encapsulation efficiency. Three main process parameters that influence encapsulation efficiency including lecithin / cholesterol mass ratio,lecithin / juglone mass ratio and injection speed were optimized by response surface analysis based on a three-variable and three-level Box-Behnken experimental design. The optimal conditions for preparing juglone liposomes were determined as 4. 86∶1. 00 of lecithin /cholesterol mass ratio,10. 92∶1. 00 of lecithin / juglone mass ratio and 1. 52 mL·min^- 1of injection speed. Under these conditions,the actual encapsulation efficiency of juglone liposomes was 79. 80%,which showed a relative error of 0. 033% when compared with the predictive value.

关 键 词：胡桃醌 脂质体 响应面分析法 理化性质 

分 类 号：TQ244.6[化学工程—有机化工]