c-Jun在鼻咽癌组织中的表达以及对鼻咽癌复发的影响  

Expression of c-jun in nasopharyngeal carcinoma and the effects on the recurrent nasopharyngeal carcinoma

作　　者：方敏[1] 宋涛[1] 吴式 吴树强[1] 贾勇士[1] 

机构地区：[1]浙江省人民医院放疗科,浙江杭州310014 [2]杭州市肿瘤医院放疗科,浙江杭州310002

出　　处：《中国医药导报》2015年第31期35-39,43,F0003,共7页China Medical Herald

摘　　要：目的 分析c-Jun在鼻咽癌组织中的表达以及对鼻咽癌复发的影响。方法 选择2003年1月-2005年9月在温州医学院附属第一医院耳鼻喉科行放疗的4例复发鼻咽癌（复发鼻咽癌组）和4例初发鼻咽癌患者（初发鼻咽癌组）的组织标本,应用磷酸化抗体蛋白质芯片筛选初发鼻咽癌组与复发鼻咽癌组患者鼻咽癌组织中的差异磷酸化蛋白,并进行聚类。并选取其中的差异磷酸化蛋白进行免疫组化试验验证,用于免疫组化验证的初发及复发鼻咽癌组织40例取自2003年8月-2010年5月温州医学院附属第一医院病理科,将其分为初发鼻咽癌组（n=20）和复发鼻咽癌组（n=20）。结果 在复发鼻咽癌组和初发鼻咽癌组中筛选出包括c-Jun（P=0.030）、Histone H2A（P=0.044）、SEK1（P=0.048）、KIT（P=0.042）、ATP1A1（P=0.007）和Synapsin（P=0.023）共6个差异蛋白,复发鼻咽癌组中Histone H2A（上调比率为1.08）、SEK1（上调比率为1.14）、KIT（上调比率为1.22）、ATP1A1（上调比率为1.22）和Synapsin（上调比率为1.22）磷酸化水平较初发鼻咽癌组患者上调,复发鼻咽癌组中c-Jun磷酸化水平较初发鼻咽癌组患者下调（下调比率为0.87）。其中c-Jun、Histone H2AX、SEK1及KIT蛋白质的磷酸化水平改变可能与鼻咽癌复发机制有关。通过免疫组化验证了c-Jun在复发鼻咽癌组低于初发鼻咽癌组,差异有统计学意义（P=0.048）。结论 磷酸化c-Jun在复发鼻咽癌组织中低表达,c-Jun磷酸化涉及调控基因、抑制细胞凋亡、促细胞增殖等信号转导通路,可能与肿瘤复发有关,还可提示预后及提供治疗靶点。Objective To analyze expression of c-jun in nasopharyngeal carcinoma and the effects on the recurrent nasopharyngeal carcinoma. Methods Four primary nasopharyngeal carcinoma tissues（p NPC group） and four relapsed nasopharyngeal carcinoma tissues（r NPC group） were obtained from the Department of Otolaryngology,the First Affiliated Hospital of Wenzhou Medical College. The differentially phosphorylation level of proteins in r NPC group and p NPC group were identified by Full Moon Bio Systems＇ Phospho Explorer antibody array and this was followed by cluster analysis. Immunohistochemistry was performed on some of differentially phosphorylated protein 40 cases of primary and relapsed nasopharyngeal carcinoma tissues were selected for immunohistochemical validation,from August 2003 to May2010 in Pathology Department of the First Affiliated Hospital of Wenzhou Medical College,and they were divided into primary nasopharyngeal carcinoma group（n=20） and relapsed nasopharyngeal carcinoma group（n=20）. Results The phosphorylation levels of proteins in two groups were different and six significantly differentially phosphorylated proteins were identified including c-Jun（P =0.030）,Histone H2A（P =0.044）,SEK1（P =0.048）,KIT（P =0.042）,ATP1A1（P =0.007）,and Synapsin（P =0.023）. Among them,compared to p NPC group the phosphorylation levels of five proteins（ATP1A1,Histone H2 A,KIT,SEK1 and Synapsin） in tissues of the r NPC group were increased,the up-regulated ratio was 1.08,1.14,1.22,1.22,1.22 respectively,and one protein was decreased（c-Jun）,the down-regulated ratio of c-Jun was 0.87. Some of the diferentially phosphorylated proteins including c-Jun,Histone H2 AX,SEK1 and KIT maybe play crucial roles in the recurrence of NPC. The phosphorylation levels of c-Jun was also decreased in r NPC group（P=0.048）by immunohistochemistry. Conclusion The phosphorylation levels of c-Jun is decrease in tissues of r NPC,which maybe play crucial roles in the recurrence of NPC. The phosphorylated c-Ju

关 键 词：鼻咽肿瘤 复发 C-JUN 磷酸化芯片 

分 类 号：R730.5[医药卫生—肿瘤]