兔眼玻璃体切除联合C_3F_8填充术后晶状体氧化损伤的研究  

Research of T-SOD and MDA in rabbit lens at the early period after vitrectomy combined with nonexpansile perfluoropropane gas

作　　者：杨艳芳 贾松柏[2] 唐罗生[2] 

机构地区：[1]重庆新视界眼科医院,中国重庆市400041 [2]中南大学湘雅二医院眼科,中国湖南省长沙市410011

出　　处：《国际眼科杂志》2015年第11期1873-1876,共4页International Eye Science

摘　　要：目的:通过对术后不同时期晶状体内总超氧化物歧化酶(T-superoxide dismatase,T-SOD)及丙二醛(malondialdehyde,MDA)含量的检测,以探讨兔眼玻璃体切除联合非膨胀浓度全氟丙烷(C3F8)填充术后早期晶状体的损伤改变是否与氧化损伤有关。方法:健康新西兰白兔45只分为三组:正常组、对照组和实验组。对照组行单纯玻璃体切除联合平衡盐溶液(BSS)填充术;实验组行玻璃体切除联合非膨胀浓度C3F8填充术。在术后1、3、8、35、45d分别将三组兔眼晶状体取出制成组织匀浆,采用分光光度法分别检测TSOD和MDA的含量。结果:T-SOD活力:正常组最高,实验组最低,三组不同时期两两相互比较均有显著统计学差异(P<0.01)。MDA含量:正常组最低,实验组最高,三组不同时期两两相互比较除术后第1d实验组与对照组比较无显著统计学差异外,其余各组两两相互比较均有显著统计学差异(P<0.01)。结论:兔眼玻璃体切除联合非膨胀浓度C3F8气体填充术后早期晶状体混浊可能与氧化损伤有关。AIM：To study the relationship between oxidative damage and the early pathological changes in rabbit lens after vitrectomy combined with nonexpansile perfluoropropane gas,by detecting T-SOD activity and MDA content at different stages after the operation.METHODS：Fourty five healthy new Zealand rabbits were divided randomly to three groups：normal group,control group and experimental group.The control group was just performed vitrectomy with BSS;the experimental group was performed vitrectomy combined with nonexpansile perfluoropropane gas.The lens were made into tissue homogenates to detect T-SOD activity and MDA content by using spectrophotometry on the1 st 3th 3th 35 th and 45 th day after the operation.RESULTS：T-SOD activity：that in normal group was the hightest,that in experimental group was the lowest,and there was significant difference（P〈0.01） among three groups in every periods after operation.MDA content：that in normal group was the lowest,that in experimental group was the hightest,and there was significant difference（P〈0.01） among three groups after the operation except the 1st day.CONCLUSION：Oxidative damage mechanism may be involved in the early damge of rabbit lens after vitrectomy combined with nonexpansile perfluoropropane gas.

关 键 词：玻璃体切除术 全氟丙烷 白内障 氧化损伤 

分 类 号：R779.6[医药卫生—眼科]