外湿对大鼠各肠段菌群的影响  

Effects of exopathic dampness on intestinal flora of rat

作　　者：王菁 刘晓燕[2] 黄晓宇[2] 

机构地区：[1]北京市宣武中医医院,北京100050 [2]北京中医药大学基础医学院,北京100029

出　　处：《吉林中医药》2015年第10期1046-1049,共4页Jilin Journal of Chinese Medicine

基　　金：国家科技支撑计划课题(2012BAJ02B05);国家自然科学基金资助项目(81001482)

摘　　要：目的通过人工控制湿度,观察大鼠空肠、回肠和结肠的大肠杆菌、乳酸杆菌和双歧杆菌的菌落数,进而阐述外湿对肠道的影响,为湿邪致病阐明机制。方法将SD雄性大鼠随机分为常湿组、高湿组、低湿组,饲养21 d后,取空肠、回肠和结肠,分别进行大肠杆菌、乳酸杆菌和双歧杆菌平板计数。结果高湿组大鼠的空肠〔(4.50±0.09)log N/m L〕和结肠中大肠杆菌菌落值〔(7.30±0.38)log N/m L〕明显升高(均为P<0.05);低湿组大鼠空肠乳酸杆菌明显增多〔(6.09±0.13)log N/m L〕,P<0.01;低湿组空肠、回肠和结肠的双歧杆菌均非常明显的增多〔(5.86±0.31),(5.94±0.33),(7.07±0.16)log N/m L〕,均为P<0.01;高湿组结肠双歧杆菌的菌落值较为明显的升高〔(6.60±0.22)log N/m L〕,P<0.05。结论外湿对机体肠道内环境有明显的影响作用,改变了肠道菌群结构,从而导致疾病的产生。Objective To obverse the number of E. coil, lactobacillus, bifidobacterium in jejunum,ileum and colon by controlling the relative humidity, and then to reveal the effect of exopathic dampness towards intestinal tract. Methods The mice were divided into normal temperature normal humidity environment （NNE）, normal temperature high hu- midity environment （NHE） ,and normal temperature low humidity environment （NLE） group ,6 mice in each group. After 21 days feed,5 cm samples were taken out from jejunum, ileum and colon respectively, and then put the three samples in 4ml normal saline. Finally test the number of E. coil, LBA and BFB in the three samples by using plate count method. Results The numbers of jejunum [ （4.50 ± 0.09） logN/mL] and colon E. coil [ （7.30 ± 0.38） logN/mL] in NHE group were obviously higher than those of NNE group （ P 〈 0.05 ）. The number of jejunum lactoba- cillus in NLE group was much increased,compared with that of NNE group [ （6.09±0. 13） logN/mL] ,P 〈0.01. The numbers of jejunum [ （5.86±0. 31 ） logN/mL] ,ileum [ （5.94 ±0.33） logN/mL3 and [ （7.07 ±0. 16） logN/mL] were obviously higher in NLE group than those of NNE group （P 〈0. 01 ）. The number of colon bifidobacterium in NLE group was higher, contrasted with that of NNE group [ （6. 60 ± 0.22） logN/mLl, P 〈 0. 05. Conclusion Exopathic dampness impacted obviously on intestinal environment, change the structure of intestinal flora to cause disease.

关 键 词：外湿 中医脾脏 肠道菌群 大鼠 

分 类 号：R223.1[医药卫生—中医基础理论]