子宫内膜癌中TβRⅠ和TβRⅡ基因甲基化状态  

The methylation status of TβRⅠ,Ⅱgene in endometrial cancer

作　　者：闫香庭[1] 刘盼[1] 蔺会兰[1] 

机构地区：[1]河北省石家庄市第一医院,050011

出　　处：《河北医药》2015年第21期3215-3218,共4页Hebei Medical Journal

摘　　要：目的探讨TβRⅠ和TβRⅡ基因甲基化与子宫内膜癌发生发展的关系。方法应用改进的甲基化特异性聚合酶链式反应(MSP)技术检测60例子宫内膜癌组织及60例癌旁正常组织中TβRⅠ、TβRⅡ基因启动子甲基化情况,分析两基因启动子甲基化与各临床指标之间的关系。结果 TβRⅠ在子宫内膜癌组和癌旁正常组的甲基化率分别为75.0%(45/60)和45.0%(27/60),子宫内膜癌组的甲基化率显著高于癌旁正常组(χ2=11.250,P=0.001)。TβRⅡ在子宫内膜癌组和癌旁正常组的甲基化率分别为68.3%(41/60)和36.7%(22/60),子宫内膜癌组的甲基化率显著高于癌旁正常组(χ2=12.063,P=0.000)。两基因启动子甲基化与临床指标差异无统计学意义(P>0.05)。结论 TβRⅠ和TβRⅡ基因启动子甲基化可能与子宫内膜癌的发生有关。Objective To investigate the correlation between methylation of transforming growth factor β receptor Ⅰ,Ⅱ（ TβRⅠ,Ⅱ） gene and pathogenesis,development of endometrial cancer（ EC）. Methods The modified methylation specific PCR（ MSP） was used to detect the methylation status of TβRⅠ,Ⅱgene promoter in 60 cases of EC tissues and 60 cases of adjacent non-cancerous tissues. The correlation between the methylation of two gene promotors and clinical indexes was analyzed. Results The mathylation rate of TβRⅠ in EC tissues and adjacent non-cancerous tissues was 75%（ 45 /60）and 45%（ 27 /60）,respectively. The methylation rate of EC tissues was significantly higher than that of adjacent non-cancerous tissues（ χ2= 11. 25,P〈0. 01）. The mathylation rate of TβRⅡ in EC tissues and adjacent non-cancerous tissues was 68. 3%（ 41 /60） and 36. 7%（ 22 /60）,respectively. The methylation rate of EC tissues was significantly higher than that of adjacent non-cancerous tissues（ χ2= 12. 06,P〈0. 01）. However there was no correlation between the methylation of two gene promotors and clinical indexes（ P〉0. 05）. Conclusion The methylation of TβR Ⅰ and TβR Ⅱpromoters may be correlated to the pathogenesis of EC.

关 键 词：子宫内膜癌 甲基化 TβRⅠ TβRⅡ 

分 类 号：R737.33[医药卫生—肿瘤]