含铜量不同的钛合金生物相容性的研究  

The influence of Cu content on the cell biocompatibility of Ti- Cu sintered alloys

作　　者：郑兰兰[1] 马志玲[1] 刘杰[2] 

机构地区：[1]佳木斯大学附属第二医院,黑龙江佳木斯154002 [2]青岛大学附属第一医院,山东青岛266003

出　　处：《黑龙江医药科学》2015年第5期58-60,62,共4页Heilongjiang Medicine and Pharmacy

摘　　要：目的:探究四组含铜量分别为2wt%、5wt%、10wt%、25wt%的Ti-Cu合金对MG-63成骨细胞的早期黏附、增殖和分化的影响。方法:扫描电镜下观察细胞的黏附情况;激光共聚焦下对细胞的早期黏附进行计数;CCK8法测细胞的增殖情况;对硝基苯磷酸盐法检测碱性磷酸酶(ALP)的活性。结果:细胞在四组材料表面生长状态良好,呈梭形,细胞饱满,有伪足伸出;细胞的增殖率和ALP的活性与纯钛没有明显的差别。结论:含铜量不同的四组材料的生物相容性良好,对细胞的黏附、增殖、分化没有不良的影响。Objective： To explore the influence of Ti- Cu alloy in four groups of copper content is respectively2 wt %,5 wt. %,10 wt. %,25 wt. % on MG- 63 early osteoblast adhesion,proliferation and differentiation.Methods： Cell adhesion was observed under scanning electron microscopy（ SEM）. Under the laser confocal early adhesion of cells to count. CCK8 was used to measure cell proliferation; P- nitrophenyl phosphate method to detect the activity of alkaline phosphatase（ ALP）. Results： Cell growth in four groups of material surface was in a good condition,and in a spindle,full,cellular pseudopod extend. Cell proliferation rate and the activity of ALP and pure titanium had no obvious difference. Conclusion： The four groups of copper content in different materials are of good biocompatibility. There is no adverse effects on cell adhesion,proliferation and differentiation,without adverse effects.

关 键 词：生物相容性 Ti-Cu合金 黏附 增殖 分化 

分 类 号：R783.1[医药卫生—口腔医学]