硅烷化表面海洋细菌对厚壳贻贝稚贝附着的影响  

Effects of marinebacteria from silanizing surfaces on plantigrade settlement of the mussel Mytilus coruscus

作　　者：孙俊杰[1] 张显[1] 郭行磐 陈芋如 沈和定[1] 丁德文[2] 杨金龙[1,2,3] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 [2]国家海洋局第一海洋研究所,山东青岛266061 [3]浙江海洋高效健康养殖协同创新中心,宁波大学,浙江宁波315211

出　　处：《水产学报》2015年第10期1530-1538,共9页Journal of Fisheries of China

基　　金：国家自然科学基金(41476131);上海市教委创新重点项目(14ZZ143);上海高校水产学一流学科建设项目

摘　　要：为探讨厚壳贻贝稚贝对自然微生物膜中海洋细菌的附着行为反应,研究了厚壳贻贝稚贝附着与硅烷基化附着基表面、微生物膜密度以及细菌种属系统发育之间的相互关系。结果发现所测海洋细菌均能显著促进厚壳贻贝稚贝的附着;其中Staphylococcus sp.1和Cobetia sp.1表现出较低诱导活性,且这两株细菌形成的微生物膜密度与稚贝附着率之间无显著相关性;其他7株海洋细菌均表现出中等程度诱导活性,且所形成的微生物膜密度与稚贝附着率之间呈显著相关性。系统发育分析表明所测海洋细菌对厚壳贻贝稚贝附着的诱导活性与细菌种属无关。研究表明,硅烷基化表面的海洋附着细菌对厚壳贻贝附着有着显著性促进作用,本研究将为后续开展厚壳贻贝稚贝附着机制奠定基础。To understand plantigrade settlement of the mussel Mytilus coruscus in response to the different bacterial strains isolated from silanizing surfaces,the relationship between bacterial biofilms and plantigrade settlement was conducted. All test marine bacteria were isolated from the biofilms formed on the silanizing surfaces and identified by 16 S r DNA gene sequences. In the laboratory,the effects of monospecific bacterial biofilms on settlement of M. coruscus plantigrades were also investigated. The phylogenetic tree derived from the elected 16 S r DNA sequence was constructed and the genetic distance was calculated. The results showed that the density of monospecific bacterial biofilms increased with the initial incubation density and all test bacterial biofilms showed significantly inducing activity on settlement of M. coruscus plantigrades. Among the 9 bacterial species,the biofilms of Staphylococcus sp. 1 and Cobetia sp. 1 induced the lower percentage of plantigrade settlement of 47% and 43%,and there was no signification correlation between the settlement and biofilm density. The biofilms of other 7 bacteria showed moderate inducing activity,and significant correlation between cell densities of these 7 bacterial strains and inducing activity were observed. The inducing activity of individual bacterial isolates was not correlated with their phylogenetic relationship. Thus,marine bacteria derived from biofilms developed on silanizing surfaces can promote the settlement of plantigrades of the mussel M. coruscus. Those results will provide some useful information to understand the relationship between bacterial biofilms and the settlement mechanism of M. coruscus plantigrades.

关 键 词：厚壳贻贝 稚贝 附着 硅烷基化 海洋细菌 

分 类 号：S968.3[农业科学—水产养殖]