细胞周期蛋白激酶抑制因子p21和p27在兔视网膜中的表达与细胞增生的关系  

Expressions of cyclin dependent kinase inhibitors p21 and p27 in rabbit retina and their association with proliferation

作　　者：王晓辉[1] 谢茂松[1] 陈鲤敏[1] 徐国兴[1] 

机构地区：[1]福建医科大学附属第一医院眼科,福州350005

出　　处：《中华实验眼科杂志》2015年第11期969-973,共5页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金项目（81271026）;福建省科技平台建设基金项目（2010Y2003）;福建医科大学苗圃科研基金项目（2010MP014）

摘　　要：背景细胞周期调节不平衡可诱发增生性玻璃体视网膜病变（PVR），研究周期蛋白依赖性激酶（CDK）及其抑制因子（CKI）对PVR形成过程中视网膜色素上皮（RPE）细胞和成纤维细胞病理增生过程中细胞周期的调控对PVR的防治具有重要意义。目的研究CKIp21和p27在不同发育阶段正常兔视网膜中的表达规律，探讨p21和p27表达与细胞生长的关系。方法按照兔龄将9只新西兰白兔分为10周龄组、20周龄组和30周龄组，每组3只。实验兔处死后取出双眼分别用于p21和p27在转录水平和翻译水平表达的检测。分离视网膜组织，采用real-timePCR和Westernblot法分别检测不同周龄兔视网膜中p21和p27mRNA及其蛋白的表达变化。结果10周龄组、20周龄组和30周龄组兔视网膜组织中p21mRNA的相对表达量分别为1．631±0．063、1．506±0．012和1．585±0．015，p27mRNA的相对表达量分别为1．581±0．048、1．470±0．012和1．490±0．013，总体比较差异均有统计学意义（p21：F=9．311，P=0．014；p27：F=12．360，P=0．007），其中10周龄组和30周龄组兔视网膜组织中p21和p27mRNA的表达量均明显高于20周龄组，差异均有统计学意义（均P〈0．05）。10周龄组、20周龄组和30周龄组兔视网膜组织中p21蛋白的相对表达量分别为0．675±O．061、0．089±0．001和0．200±0．007，p27蛋白的相对表达量分别为0．928±O．019、0．183±0．005和0．576±0．089，总体比较差异均有统计学意义（p21：F=228．905，P〈0．001；p27：F=148．957，P〈0．001），其中10周龄组和30周龄组兔视网膜组织中p21和p27蛋白的表达量均明显高于20周龄组，差异均有统计学意义（均P〈0．01）。兔视网膜中p21与p27间mRNA及蛋白的相对表达量均呈正相关（mRNA：r=0．906，P〈0．01；蛋白：r=0．913，P〈0．01）。结论p21和p27在生长发育期的兔视网膜中表达量升高，随着生长过程变缓其Background The imbalance of cell cycle regulation results in proliferative vitreoretinopathy （ PVR）. Studing the effects of cyclin dependent kinase （CDK） and CDK inhibitor （CKI） on the cell cycle regulation of retinal pigment epithelial （RPE） cells and fibroblasts in PVR formation is of important significance. Objective This study was to investigate the expressing trend of p21 ,p27 and CDK inhibitors in retinas of different ages of rabbits and explore the relationship between p21 or p27 and cell growth. Methods Nine clean New Zealand rabbits were assigned to 10-week group ,20-week group and 30-week group according to the age and 3 rabbits for each. The eyeballs were enucleated binocularly after the animals were sacrificed and retinas were isolated. Real-time PCR and Western blot were employed to detect the expressions of p21 and p27 mRNA and their proteins in retinas of the rabbits. The use and care of the animals followed the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Committee. Results The relative expressing levels of p21 mRNA were 1. 631± 0. 063,1. 506±0. 012 and 1. 585±0. 015,and those of p27 mRNA were 1. 581±0. 048,1. 470±0. 012 and 1. 490±0. 013 in the 10-week group,20-week group and 30-week group, respectively, showing significant differences among the groups （p21 mRNA： F=9.311,P=0.014;p27 mRNA： F=12.360,P=0.007）,and the p21 and p27 mRNA expressing levels were significantly higher in the 10-week group and 30-week group than those in the 20-week group （ all at P〈 0. 05 ）. The expressing levels of p21 protein were 0. 675± 0. 061,0. 089 ±0. 001 and 0. 200± 0. 007, and those of p27 protein were 0. 928＋0. 019,0. 183±0. 005 and 0. 576＋0. 089 in the 10-week group,20-week group and 30-week group,respectively, with remarkable differences among the groups （ p21 ： F = 228. 905, P〈0. 001 ; p27 ： F = 148.957,P〈0.001 ）, and the expressions were significantly raised in the 10-week group and 30-week g

关 键 词：视网膜 视网膜色素上皮/细胞学 增生 周期蛋白依赖性激酶/代谢 周期蛋白依赖性激 酶抑制因子/代谢 细胞周期 兔 

分 类 号：R774.1[医药卫生—眼科]