KiSS-1在卵巢癌免疫学诊断中的价值  

The value of KiSS-1 in immunodiagnosis of ovarian cancer

作　　者：李观华[1,2] 潘志雄[1,2] 刘晓峰[1,2] 陈雪礼[1,2] 薛国辉[1,2] 

机构地区：[1]九江市第一人民医院 [2]南昌大学附属九江医院,检验科332000

出　　处：《实验与检验医学》2015年第5期592-595,共4页Experimental and Laboratory Medicine

基　　金：江西省卫生计生委科技计划(项目合同编号:20157063);九江市卫生计生委科技计划(项目合同编号:20141605)

摘　　要：目的探讨KiSS-1在卵巢癌免疫学诊断中的临床价值。方法前期研究中通过克隆和免疫动物提纯出兔抗人KiSS-1抗体,将纯化的兔抗人KiSS-1抗体进行包被制成ELISA板,采用ELISA双抗体夹心法检测卵巢癌患者的血清KiSS-1浓度;同时采用化学发光法检测卵巢癌患者、卵巢良性肿瘤患者及同期健康对照人群血清中CA-125、CA-199、CEA水平。评价KiSS-1作为卵巢癌诊断指标的可能性以及联合检测时对卵巢癌诊断率的影响。结果 KiSS-1、CA-125、CA-199、CEA在卵巢癌患者血清中的检测阳性率明显高于卵巢良性肿瘤患者(P<0.05),其中CA-125最高为79.03%,KiSS-1较低为48.93%。四种标志物特异性水平均在75%以上,KiSS-1为79.55%,高于CA-125。KiSS-1的诊断准确性与CEA相当,联合Youden指数分析作为单个诊断指标,KiSS-1与CEA对卵巢癌的诊断能力相当。联合诊断时,KiSS-1+CA125+CA199+CEA组除特异性外诊断灵敏度、准确性及Youden指数均最高,加入KiSS-1后提升了CA125+CA199+CEA原有组合的诊断能力。结论 KiSS-1联合CA-125、CA-199、CEA可提升卵巢癌诊断准确性,因而或可成为卵巢癌免疫学诊断中具有重要临床价值的指标。Objective To investigate the value of KiSS-1 in immunodiagnosis of ovarian cancer. Methods In our previous study, we have purified rabbit anti-human KiSS-1 antibody and KiSS-1 protein through gene cloning and immunizing rabbit. Then the ELISA method was established to detect serum KiSS-1 concentration. In this research, KiSS-1 levels in ovarian cancer group,benign ovarian tumor group and normal control were measured by the established ELISA. CA-125, CA-199 and CEA were detected by chemiluminescence method. Results The sensitivity of CA-125, CA-199, CEA and KiSS-1 in ovarian cancer patients was significantly higher than that in benign ovarian tumor group and normal control（P〈0.05）. The highest sensitivity of 79.03% was possessed by CA-125, while the sensitivity of KiSS-1 was relatively low（48.93%）. These four kinds of ovarian cancer biomarkers were all high specificity, including KiSS-1 which specificity was higher than CA-125. The diagnostic ability of KiSS-1 and CEA was approximately similar when analyzing combined with accuracy and Youden Index. combined detection of KiSS-1, CA125,CA199 and CEA is considered the best since the sensitivity, accuracy and Youden Index were all highest. When KiSS-1 was added to present diagnosis group（CA-125＋CA-199＋CEA）, the new group harbored high accuracy for diagnosing ovarian cancer.Conclusion Detection of serum KiSS-1 is helpful in diagnosis of ovarian cancer. Combined detection of KiSS-1, CA-125, CA-199 and CEA could improve sensitivity, specificity, accuracy in diagnosis of ovarian cancer, and thus KiSS-1 might be a good auxiliary immunodiagnosis marker in ovarian cancer.

关 键 词：KISS-1 卵巢癌 免疫学 价值 

分 类 号：R737.31[医药卫生—肿瘤] R446.62[医药卫生—临床医学]