β-榄香烯对人胃癌细胞SGC7901作用的蛋白质组学研究  

A proteomic analysis of effects ofβ-elemene on human gastric cancer cell line SGC7901

作　　者：刘俊松[1] 车向明[1] 仇广林[1] 樊林[1] 赵伟[1] 贺仕才[1] 常帅[1] 王曙逢[1] 

机构地区：[1]西安交通大学医学部第一附属医院普外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2015年第6期840-844,861,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81172357)~~

摘　　要：目的探讨β-榄香烯对胃癌细胞作用的蛋白质分子基础。方法采用MTT法观察β-榄香烯对SGC7901胃癌细胞活性的影响;iTRAQ蛋白质组学筛选β-榄香烯作用SGC7901胃癌细胞蛋白质表达变化;Western blot对所筛选差异表达蛋白质进行验证。结果β-榄香烯显著抑制SGC7901胃癌细胞活性;β-榄香烯干预引起SGC7901细胞147个蛋白质表达上调,86个蛋白质表达下调。Western blot对PAK1IP1(p21-activated protein kinase-interacting protein 1)、BTF(Bcl-2-associated transcription factor 1)和TOPIIα(topoisomerase 2-alpha)表达验证显示,PAK1IP1和BTF表达上调,TOPIIα表达下调,与蛋白质组学结果一致。筛选出的差异表达蛋白所涉及信号通路主要包括核糖体信号通路、过氧化物酶体增殖物活化受体信号、细胞骨架肌动蛋白调节信号、细胞吞噬以及氨基酸的合成代谢。结论β-榄香烯对胃癌细胞具有显著的抑制作用,筛选的差异表达蛋白对研究β-榄香烯抗肿瘤作用机制具有重要的参考价值。Objective To investigate the effect ofβ-elemene on SGC7901 gastric cancer cell line and the potential proteins involved. Methods Human SGC7901 gastric cancer cells were treated with different concentrations ofβ-elemene.Cell viability was assessed.A proteomic method,isobaric tags for relative and absolute quantitation（iTRAQ）,was employed to detect the proteins altered byβ-elemene.Protein expression was validated by Western blot.Results β-elemene inhibited the viability of SGC7901 gastric cancer cells in a dose-dependent manner.Altogether,147 upregulated proteins and 86 downregulated proteins were identified in response toβ-elemene treatment in SGC7901 gastric cancer cell line.Among them,the expressions of p21-activated protein $kinase-interacting protein 1（PAK1IP1）,Bcl-2-associated transcription factor 1（BTF）and topoisomerase 2-alpha（TOPIIα）were validated by Western blot and the trends were consistent with iTRAQ results.Top pathways involved inβ-elemene treatment in SGC7901 gastric cancer cell line included ribosome signaling,peroxisome proliferatoractivated receptors（PPARs）signaling pathway,regulation of actin cytoskeleton,phagosome,biosynthesis and metabolism of some amino acids.Conclusion Our results suggest a promising therapeutic role ofβ-elemene for gastric cancer.The differentially expressed proteins give us better insights into the potential mechanisms involved in gastric cancer treatment usingβ-elemene.

关 键 词：Β-榄香烯 胃癌 增殖 蛋白质组学 PAK1IP1 TOPIIα 

分 类 号：R735.2[医药卫生—肿瘤]