血浆和组织miR-124表达与大肠癌患者预后的相关性分析  

Expression levels of microRNA-124 in plasma and tissure correlated with prognosis in patients with colorectal cancer

作　　者：吕梅[1] 宦欣 李芊蔚[1] 王砚砚[1] 

机构地区：[1]大连大学附属中山医院消化内科,116001

出　　处：《疑难病杂志》2015年第11期1125-1128,共4页Chinese Journal of Difficult and Complicated Cases

摘　　要：目的分析血浆和组织中miR-124表达水平与大肠癌患者预后的相关性。方法收集大肠癌患者71例,其中无法切除患者49例、完全切除患者22例,收集其血浆和肿瘤组织样本。检测肿瘤无法切除患者血浆和组织中miR-101、miR-124、miR-126、miR-210、miR-217的表达,通过实时定量反转录聚合酶链反应（qRT-PCR）分析miR-124表达和患者及无进展生存期（PFS）的相关性。结果 qRT-PCR检测无法切除大肠癌患者血浆和组织中miRNAs表达,其中高表达的miR-124变化最显著（分别为P〈0.05,P〈0.01）,其他miRNAs无明显变化。血浆中miR-124与患者OS相关：在无法切除大肠癌患者血浆、组织中高表达miR-124与OS呈正相关（血浆：OR=0.451,95%CI0.189~0.654,P=0.002;组织：OR=0.295,95%CI 0.493~1.374,P=0.037）。Cox比例风险模型评估：采用Cox比例风险模型评估发现血浆中高表达miR-124与PFS相关（OR=0.147,P=0.022）;而组织中高表达miR-124与PFS无相关性（OR=0.281,P=0.057）,血浆和组织中低表达miR-124与低死亡风险比无相关性（OR=2.07,P=0.163）。高miR-124表达同PFS无相关性（OR=1.85,P=0.725）。结论血浆中miR-124表达可以作为评价大肠癌预后的生物标记物。Objective To analyze the correlation between plasma and tissue miR-124 expression and overall survival（OS） of patients with colorectal cancer.Methods Collected 71 cases of patients with colorectal cancer which cannot be removed（n=49）,completely resected（n =22）,collected the plasma and tumor tissue samples.Detected tumor unresectable tissue and plasma of miR-101,miR-124,miR-126,miR-210,miR-217＇s expression,by quantitative real-time reverse transcription polymerase chain reaction（qRT-PCR） to analyze the correlation between miR-124 expression and the prognosis of the patients.Results qRT-PCR for detection of unresectable expression of miRNAs in plasma and tissue in patients with colorectal cancer,high expression of miR-124＇s changes revealed as the most significant（P〈0.05.P〈0.01）,and other miRNAs did not show significant changes.（1） Correlation between plasma miR-124 and patients＇ OS：in unresectable colorectal cancer patients,plasma and tissue expression of miR-124 was positively correlated with the OS（OR =0.451,95%CI0.189- 0.654,P =0,002;OR =0.295,95%CI 0.493- 13.74.P =0.037）.（2） Cox proportional hazards models assessment：the Cox proportional hazards model evaluation revealed that the high expression of miR-124 in plasma were correlated with PFS（OR = 0.147,P a 0.022）;and high expression of miR-124 in tissue had no correlation with PFS（OR =0.281,P =0.057）,plasma and tissue low expression of miR-124 had no correlation with low mortality risk ratio（OR =2.07,P =0.163）.High expression of miR-124 had no correlation with PFS（OR = 1.85,P =0.725）.Conclusion It demonstrated that the plasma miR-124 expressions could he used as biological markers for evaluating the prognosis of colorectal cancer.

关 键 词：大肠癌 miR-124 总生存期 相关性 

分 类 号：R735.34[医药卫生—肿瘤]